INTRODUCTION M2 macrophages are predominant in the immune infiltrates of resected tumours, but little is known about macrophage phenotype in the local lung cancer environment, which may be evaluated by bronchoalveolar lavage fluid (BALF). AIM OF THE STUDY To find differences between BALF from lung affected by cancer (clBALF) and hlBALF from the opposite, healthy lung, as a control, from the same patient, regarding their individual macrophage polarization and their correlation with IL-10 and TGF-β. MATERIAL AND METHODS Eighteen patients with confirmed lung cancer were investigated. Macrophage subtyping was performed by immunofluorescence with antibodies anti-CCR7 and CD163 (M1 and M2, respectively). RESULTS We found five populations of macrophages: cells with a single reaction: only for CCR7+ or CD163+, a double reaction (CCR7+CD163+), cells with a stronger CD163 (CCR7lowCD163+), and cells with a stronger CCR7 (CCR7+CD163low). The main population in the clBALF was composed of cells with a phenotype similar to M2 (CCR7lowCD163+), while in the hlBALF the predominating phenotype was the one similar to M1 (CCR7+CD163low). The median proportion of TGF-β1 concentration was higher in the clBALF and hlBALF supernatant than in the serum. CONCLUSIONS In this study we confirmed the usefulness of the immunofluorescence method with CCR7 and CD163 in the evaluation of BALF macrophage polarization in lung cancer.

中文翻译：

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CD163 和 CCR7 作为肺癌微环境中巨噬细胞极化的标志物。

引言 M2巨噬细胞在切除肿瘤的免疫浸润中占主导地位，但对局部肺癌环境中的巨噬细胞表型知之甚少，可通过支气管肺泡灌洗液（BALF）进行评估。研究目的 发现来自受癌症影响的肺的 BALF (clBALF) 和来自对侧健康肺的 hlBALF 作为对照，来自同一患者，关于它们的个体巨噬细胞极化及其与 IL-10 和 TGF- β。材料和方法 对 18 名确诊为肺癌的患者进行了调查。使用抗 CCR7 和 CD163 抗体（分别为 M1 和 M2）通过免疫荧光进行巨噬细胞亚型分型。结果 我们发现了五个巨噬细胞群： 单一反应的细胞：仅针对 CCR7+ 或 CD163+，双重反应（CCR7+CD163+），具有更强 CD163 的细胞 (CCR7lowCD163+) 和具有更强 CCR7 的细胞 (CCR7+CD163low)。clBALF 中的主要细胞群由表型类似于 M2 (CCR7lowCD163+) 的细胞组成，而 hlBALF 中的主要表型是类似于 M1 (CCR7+CD163low) 的细胞。clBALF和hlBALF上清液中TGF-β1浓度的中位比例高于血清。结论 在本研究中，我们证实了使用 CCR7 和 CD163 免疫荧光法评估肺癌中 BALF 巨噬细胞极化的有效性。clBALF和hlBALF上清液中TGF-β1浓度的中位比例高于血清。结论 在本研究中，我们证实了使用 CCR7 和 CD163 免疫荧光法评估肺癌中 BALF 巨噬细胞极化的有效性。clBALF和hlBALF上清液中TGF-β1浓度的中位比例高于血清。结论 在本研究中，我们证实了使用 CCR7 和 CD163 免疫荧光法评估肺癌中 BALF 巨噬细胞极化的有效性。