BACKGROUND A burn wound is one of the most frequent and devastating injuries for patients which requires extensive care. Early treatment of burn wounds improves healing significantly. OBJECTIVE This study was designed to investigate the efficacy of amnion and collagen-based hydrogels on cutaneous burn wound healing in rats with covering membrane. METHODS We prepared a novel cell free hydrogel comprising human amnion, rabbit collagen, carboxymethyl cellulose sodium salt, citric acid, methyl paraben, propyl paraben, glycerin and triethanol amine. The wound covering membrane was developed from rabbit collagen and prawn shell chitosan. Beside swelling ratio, water absorption, equilibrium water content, gel fraction and spreadability analysis, in vitro cytotoxicity and biocompatibility tests were performed for the formulated hydrogels. Following the skin irritation study, second-degree burns were created on the dorsal region of the rats and the gels were applied with/without covering membrane to study the wound contraction and re-epithelialization period. RESULTS The formulated hydrogels were observed non-cytotoxic and compatible with human blood cells. No erythema and edema were found in skin irritation assay confirming the safety and applicability. Hydrogel consisting in a combination of amnion and collagen demonstrated significantly rapid wound healing, driven by complete re-epithelialization (16.75 ± 0.96 days) and closure by wound contraction (72 ± 3.27%, P < 0.0000009) when wound dressing membrane was used, whereas this gel alone healed about 62.5 ± 4.43% (P < 0.00001) and required 18.75 ± 0.50 days to complete re-epithelialization. Additionally, the gel with covering membrane treated group had maximum average body weight, food and water intake. CONCLUSION The amnion and collagen-based blended gel offers alternative possibilities to treat skin wounds when covered with film, which could overcome the limitations associated with modern therapeutic products such as high costs, long manufacturing times, complexities, storing, and presence of living biomaterials.

中文翻译：

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在伤口敷料生物膜存在的情况下，羊膜和胶原蛋白基混合水凝胶可改善大鼠皮肤伤口模型的烧伤愈合功效。

背景技术烧伤伤口是需要广泛护理的患者中最常见和最具破坏性的伤害之一。早期治疗烧伤创面可显着改善愈合。目的本研究旨在探讨羊膜和胶原蛋白水凝胶对覆盖膜大鼠皮肤烧伤创面愈合的作用。方法我们制备了一种新型的无细胞水凝胶，其中包含人羊膜，兔胶原蛋白，羧甲基纤维素钠盐，柠檬酸，对羟基苯甲酸甲酯，对羟基苯甲酸丙酯，甘油和三乙醇胺。伤口覆盖膜由兔胶原蛋白和虾壳壳聚糖制成。除了溶胀率，吸水率，平衡水含量，凝胶分数和铺展性分析之外，还对配制的水凝胶进行了体外细胞毒性和生物相容性测试。皮肤刺激性研究之后，在大鼠的背侧区域产生了第二度烧伤，并在有/无覆盖膜的情况下使用了凝胶，以研究伤口的收缩和重新上皮形成的时期。结果观察到配制的水凝胶无细胞毒性，并且与人血细胞相容。皮肤刺激性试验未发现红斑和水肿，证实了其安全性和适用性。当使用伤口包扎膜时，由羊膜和胶原蛋白组成的水凝胶表现出明显的快速伤口愈合，这是由于完全重新上皮化（16.75±0.96天）和伤口收缩而闭合（72±3.27％，P <0.0000009）驱动的，而仅此凝胶即可治愈约62.5±4.43％（P <0.00001），并需要18.75±0.50天才能完成上皮再生。另外，覆盖膜治疗组的凝胶具有最大的平均体重，食物和水的摄入量。结论羊膜和胶原蛋白基混合凝胶在覆盖薄膜时提供了治疗皮肤伤口的替代方法，可以克服与现代治疗产品相关的局限性，例如成本高，制造时间长，复杂性，储存性和生物材料的存在。