Research efforts of elucidating the molecular mechanisms governing heat shock response which imparts thermo-tolerance ability to indigenous breeds are very scanty. Therefore, a study was conducted with the primary objective to determine the impact of heat stress on the expression pattern of different heat shock response genes in the hepatic tissues of indigenous Salem Black goat. The study was conducted for a period of 45 days in twelve 1-year-old female Salem Black breed goats. The animals were randomly allocated into two groups of six animals each, C (n = 6; Salem Black control) and HS (n = 6; Salem Black heat stress). The C animals were maintained in the shed in comfort condition while HS animals were exposed outside to summer heat stress between 10:00 h to 16:00 h during experimental period. The animals were slaughtered at the end of study and their liver samples were collected for assessing the different heat shock response genes. Based on the results obtained from the study it was established that the heat shock protein 70 (HSP70), HSP90, super oxide dismutase (SOD), nitrous oxide synthase 1 (NOS1) genes were significantly (P < 0.05) down regulated. However, heat stress did not influence the expression pattern of heat shock factor-1 (HSF1) gene. The lower level of expression of all heat shock response genes may be due to less magnitude of heat stress in the study to induce cellular stress response in Salem Black goats.

中文翻译：

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夏季引起了塞勒姆黑山羊不同热休克反应基因定量表达模式的变化。

阐明控制热激反应的分子机制的研究工作非常少，该机制可赋予本地品种耐热性。因此，进行了一项主要目的是确定热应激对本土塞勒姆黑山羊肝组织中不同热休克反应基因表达模式的影响的研究。该研究对12只1岁雌性塞勒姆·布莱克（Salem Black）山羊进行了为期45天的研究。将动物随机分为两组，每组六只动物，C（n ＝ 6； Salem Black对照）和HS（n ＝ 6； Salem Black热应激）。在实验期间，在10：00h至16：00h之间，将C动物保持在棚屋中，使其处于舒适状态，而将HS动物暴露于夏季的高温胁迫下。在研究结束时将动物屠宰，并收集它们的肝脏样品以评估不同的热休克反应基因。根据从研究中获得的结果，可以确定热休克蛋白70（HSP70），HSP90，超氧化物歧化酶（SOD），一氧化二氮合酶1（NOS1）基因显着下调（P <0.05）。但是，热应激不影响热激因子-1（HSF1）基因的表达模式。所有热休克反应基因的较低表达水平可能是由于在诱导萨利姆黑山羊细胞应激反应的研究中热应激的幅度较小。超氧化物歧化酶（SOD），一氧化二氮合酶1（NOS1）基因显着下调（P <0.05）。但是，热应激不影响热激因子-1（HSF1）基因的表达模式。所有热休克反应基因的较低表达水平可能是由于在诱导塞勒姆黑山羊细胞应激反应的研究中较少的热应激。超氧化物歧化酶（SOD），一氧化二氮合酶1（NOS1）基因显着下调（P <0.05）。但是，热应激不影响热激因子-1（HSF1）基因的表达模式。所有热休克反应基因的较低表达水平可能是由于在诱导萨利姆黑山羊细胞应激反应的研究中热应激的幅度较小。