Abstract

An experimental evolution system with a wood-rotting microbial consortium (BOS08) has demonstrated the acquisition of a new ability to exploit a previously untapped carbon source, such as the recalcitrant carbamazepine (CBZ). The improved extraction method has provided an accurate CBZ depletion rate from BOS08 of \(2.14 \pm 0.42 \times 10^{-3}\;\text{ h }^{-1}\). The consortium did not use cometabolism to process CBZ and the intermediate metabolite produced 10,11-dihydroxycarbamazepine was not pharmacologically active and toxic. The bacteria identification by massive sequencing (Illumina) confirmed the dominance of Proteobacteria Phylum such as genera Cupriavidus sp., Sphingomonas sp., Delftia sp., Acinetobacter sp. and Methylobacterium sp. coexisting through all biodegradation process. Based on biological principles, we model the consortium-CBZ kinetics with a set of nonlinear ordinary differential equations with logistic growth type terms. The use of experimental data combined with logistic growth models allow us to test new functional features acquired by the consortium.

Graphic Abstract

摘要

具有木腐烂微生物联合体（BOS08）的实验进化系统已证明获得了利用以前未开发的碳源（如顽固的卡马西平（CBZ））的新功能。改进的提取方法从BOS08提供了准确的CBZ耗尽率\（2.14 \ pm 0.42 \乘以10 ^ {-3} \; \ text {h} ^ {-1} \）。该财团没有使用新陈代谢来加工CBZ，而产生的中间代谢物10,11-二羟基卡马西平没有药理活性和毒性。该细菌鉴定通过大规模测序（Illumina公司）证实变形菌门的优势，如属钩虫贪铜菌属，鞘氨醇单属，代尔夫特菌属，不动杆菌sp。和甲基杆菌属。在所有生物降解过程中共存。基于生物学原理，我们使用一组具有对数增长类型项的非线性常微分方程对联合体-CBZ动力学进行建模。实验数据与逻辑增长模型的结合使我们能够测试财团获得的新功能。

图形摘要