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Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.
Science Advances ( IF 11.7 ) Pub Date : 2020-04-01 , DOI: 10.1126/sciadv.aay9572
Stephen D Carter 1 , Cheri M Hampton 2 , Robert Langlois 2 , Roberto Melero 3 , Zachary J Farino 4 , Michael J Calderon 5 , Wen Li 2 , Callen T Wallace 5 , Ngoc Han Tran 6 , Robert A Grassucci 2 , Stephanie E Siegmund 7, 8 , Joshua Pemberton 9 , Travis J Morgenstern 10, 11 , Leanna Eisenman 12 , Jenny I Aguilar 10, 11 , Nili L Greenberg 10, 11 , Elana S Levy 10, 11 , Edward Yi 10, 11 , William G Mitchell 10, 11 , William J Rice 13 , Christoph Wigge 13 , Jyotsna Pilli 14 , Emily W George 4 , Despoina Aslanoglou 4 , Maïté Courel 15 , Robin J Freyberg 4 , Jonathan A Javitch 10, 11 , Zachary P Wills 12 , Estela Area-Gomez 8 , Sruti Shiva 14, 16, 17 , Francesca Bartolini 18 , Allen Volchuk 19 , Sandra A Murray 5 , Meir Aridor 5 , Kenneth N Fish 4 , Peter Walter 6 , Tamas Balla 9 , Deborah Fass 20 , Sharon G Wolf 21 , Simon C Watkins 5 , José María Carazo 3 , Grant J Jensen 22 , Joachim Frank 2, 23 , Zachary Freyberg 4, 5
Affiliation  

The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.

中文翻译:


核糖体相关囊泡:分泌细胞内质网的动态亚区室。



内质网(ER)是一个高度动态的膜网络。在这里,我们将活细胞显微镜与原位冷冻电子断层扫描相结合,直接可视化几种分泌细胞类型(包括接近天然条件下的胰腺β细胞和神经元)的内质网动态。利用这些成像方法,我们确定了一种新型的移动形式的内质网,即核糖体相关囊泡 (RAV),它主要存在于细胞外周,在不同的细胞类型和物种中是保守的。我们表明,RAV 作为独特的、高度动态的结构存在,与完整的内质网网状结构分开,通过直接的膜间接触与线粒体相互作用。这些发现描述了细胞内一个新的内质网亚区室。
更新日期:2020-04-01
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