Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) biotyping is an innovative strategy, applied successfully for the identification of numerous arthropod families including mosquitoes. The effective mosquito identification using this emerging tool was demonstrated possible at different steps of their life-cycle, including eggs, immature and adult stages. Unfortunately, for species identification by MS, the euthanasia of the mosquito specimen is required. To avoid mosquito euthanasia, the present study assessed whether aedine mosquitoes could be identified by MALDI-TOF MS biotyping, using their respective exuviae. In this way, exuviae from the fourth-instar and pupal stages of Aedes albopictus and Aedes aegypti were submitted to MALDI-TOF MS analysis. Reproducible and specific MS spectra according to aedine species and stage of exuviae were observed which were objectified by cluster analyses, composite correlation index (CCI) tool and principal components analysis (PCA). The query of our reference MS spectra database (DB) upgraded with MS spectra of exuviae from fourth-instar larvae and pupae of both Aedes species revealed that 100% of the samples were correctly classified at the species and stage levels. Among them, 93.8% (135/144) of the MS profiles reached the threshold log score value (LSV > 1.8) for reliable identification. The extension of reference MS spectra DB to exuviae from fourth-instar and pupal stages made now possible the identification of mosquitoes throughout their life-cycle at aquatic and aerial stages. The exuviae presenting the advantage to avoid specimen euthanasia, allowing to perform complementary analysis on alive mosquitoes.

中文翻译：

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的识别伊蚊通过使用从幼虫和蛹的蜕皮蛋白签名MALDI-TOF MS生物分型蚊子

基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）生物分型是一种创新策略，已成功应用于识别包括蚊子在内的许多节肢动物科。事实证明，使用这种新兴工具对蚊子进行有效识别可能在其生命周期的不同阶段，包括卵，未成熟阶段和成年阶段。不幸的是，对于通过MS进行物种识别，蚊子标本需要安乐死。为避免蚊子安乐死，本研究评估了可通过MALDI-TOF MS生物分型法使用其各自的脓疱来鉴定伊蚊。通过这种方式，来自白纹伊蚊和埃及伊蚊的四龄期和ex期的足癣被送至MALDI-TOF MS分析。根据聚类分析，复合相关指数（CCI）工具和主成分分析（PCA）的客观性，观察到根据依地犬种类和阶段的Exuviae可再现的和特定的MS光谱。我们的参考MS光谱数据库（DB）的查询升级了两个伊蚊属的四龄幼虫和p的足虫的MS谱图，发现100％的样品在物种和阶段水平上正确分类。其中，有93.8％（135/144）的MS配置文件达到了对数日志阈值（LSV> 1.8）以进行可靠识别。现在，将参考MS光谱数据库扩展到四龄期和p期的痣，使在水生和空中阶段的整个蚊子生命周期识别成为可能。exuviae具有避免标本安乐死的优势，