Oryza sativa FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (OsFIT/OsbHLH156) interacts with OsIRO2 to regulate iron homeostasis.,Journal of Integrative Plant Biology

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Oryza sativa FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (OsFIT/OsbHLH156) interacts with OsIRO2 to regulate iron homeostasis.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-05-01 , DOI: 10.1111/jipb.12933
Gang Liang _{1,

2} , Huimin Zhang ₁ , Yang Li _{1,

2} , Mengna Pu _{1,

2} , Yujie Yang _{1,

2,

3} , Chenyang Li _{1,

2,

3} , Chengkai Lu _{1,

2} , Peng Xu _{1,

2,

3} , Diqiu Yu _{1,

4}

Affiliation

Iron (Fe) is indispensable for the growth and development of plants. It is well known that FER‐LIKE FE DEFICIENCY‐INDUCED TRANSCRIPTION FACTOR (FIT) is a key regulator of Fe uptake in Arabidopsis. Here, we identify the Oryza sativa FIT (also known as OsbHLH156) as the interacting partner of IRON‐RELATED BHLH TRANSCRIPTION FACTOR 2 (OsIRO2) that is critical for regulating Fe uptake. The OsIRO2 protein is localized in the cytoplasm and nucleus, but OsFIT facilitates the accumulation of OsIRO2 in the nucleus. Loss‐of‐function mutations of OsFIT result in decreased Fe accumulation, severe Fe‐deficiency symptoms, and disrupted expression of Fe‐uptake genes. In contrast, OsFIT overexpression promotes Fe accumulation and the expression of Fe‐uptake genes. Genetic analyses indicate that OsFIT and OsIRO2 function in the same genetic node. Further analyses suggest that OsFIT and OsIRO2 form a functional transcription activation complex to initiate the expression of Fe‐uptake genes. Our findings provide a mechanism understanding of how rice maintains Fe homeostasis.

中文翻译：

水稻像铁蛋白一样，由FE缺乏诱导的转录因子（OsFIT / OsbHLH156）与OsIRO2相互作用以调节铁稳态。

铁（Fe）对于植物的生长和发育必不可少。众所周知，FER-like FE缺陷诱导转录因子（FIT）是拟南芥中铁吸收的关键调节剂。在这里，我们确定水稻稻FIT（也称为OsbHLH156）是铁相关的BHLH转录因子2（OsIRO2）的相互作用伴侣，这对调节铁的摄取至关重要。OsIRO2蛋白位于细胞质和细胞核中，但是OsFIT促进了OsIRO2在细胞核中的积累。OsFIT的功能丧失突变导致Fe积累减少，严重的Fe缺乏症状和Fe摄取基因的表达中断。相比之下，OsFIT过表达促进Fe积累和Fe摄取基因的表达。遗传分析表明，OsFIT和OsIRO2在同一遗传节点中起作用。进一步的分析表明，OsFIT和OsIRO2形成功能性转录激活复合物，以启动Fe摄取基因的表达。我们的发现为水稻如何维持铁稳态提供了一种机制理解。

更新日期：2020-05-01

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