In order to analyze the bioavailable oxytetracycline (OTC) contents of soils, a new high-efficiency and economical pretreatment method was established using a molecularly imprinted polymer (MIP) with high affinity and specific selectivity for OTC synthesized by bulk polymerization. For comparison, an Oasis HLB solid phase extraction (SPE) method was used to measure the total extractable OTC content of soils. The resulting OTC recoveries from the spiked soil solutions using the MIP method ranged from 80.78±.26% to 93.75 ± 6.79%, with an average of 86.00 ± 2.13%, and a relative standard deviation of 2.48% (n= 5), when OTC concentrations in spiked soil solutions varied from 0.5 to 100.0 μg L⁻¹. When OTC contents in spiked soils varied from 1.0 to 500.0 μg kg⁻¹ and soils were aged for three weeks, OTC recoveries from spiked soils with the Oasis HLB SPE method ranged from 78.16 ± 3.72% to 96.67 ± 1.62% (n= 3), while those with the MIP method ranged from 57.43 ± 3.48% to 76.34 ± 1.30% (n= 3). Analyses of OTC contents in five soil samples indicated that bioavailable OTC contents were only 1.35%–5.87% of the total soil OTC contents. Data from the new method were more valuable than the Oasis HLB SPE method on OTC pollution intensity assessment, potential ecological, and environmental risks evaluation.

为了分析土壤中生物可利用的土霉素含量，建立了一种新的高效经济的预处理方法，该方法利用分子印迹聚合物（MIP）对通过本体聚合合成的OTC具有高亲和力和特异性。为了进行比较，使用了Oasis HLB固相萃取（SPE）方法来测量土壤中总可萃取OTC含量。使用MIP方法从加标土壤溶液中获得的OTC回收率在80.78±.26％到93.75±6.79％之间，平均为86.00±2.13％，相对标准偏差为2.48％（n = 5）。加标土壤溶液中的OTC浓度从0.5到100.0μgL ^-1不等。当加标土壤中的OTC含量从1.0到500.0μgkg ^-1变化时并且土壤老化了三周，采用Oasis HLB SPE方​​法从加标土壤中的OTC回收率介于78.16±3.72％至96.67±1.62％（n = 3），而采用MIP方法的则介于57.43±3.48％至76.34。 ±1.30％（n = 3）。对5个土壤样品中OTC含量的分析表明，生物可利用的OTC含量仅为土壤OTC总含量的1.35％–5.87％。在OTC污染强度评估，潜在的生态和环境风险评估方面，新方法的数据比Oasis HLB SPE方​​法更有价值。