Abstract Dynamic surfaced-enhanced Raman spectroscopy (D-SERS) has been widely applied in chemical analysis due to its simple operation and high sensitivity. A new method was developed for the quantitative determination of auramine O in bean curd sheets using D-SERS. Through the optimization of measurement time interval and determination and stability and other SERS conditions, effective and stable SERS measurements of auramine O were performed during the sample solution evaporation process. Dispersive solid phase extraction was applied to remove as many impurities as possible. Following the evaluation of the spectra, the peak at 1114 cm−1 in the SERS spectrum was selected to quantify the auramine O present in the bean curd sheets. A matrix standard curve-external standard method was used to conduct the statistical analysis. The results showed that there was good linearity between the chromatographic SERS peak and the concentration of auramine O across the range from 0.5 to 10 mg/L. The correlation coefficient (R2) was greater than 0.99. At spiked levels of 0.5, 1.0, and 5.0 mg/kg, the average recoveries of auramine O in the bean curd sheets were from 92.3% to 96% and the relative standard deviations were from 4.6% to 8.3%. The lowest spiked concentration was determined to be the limit of quantitation. As a consequence, this study provides a new stable, effective, rapid approach for the quantitative determination of auramine O in bean curd sheets.

中文翻译：

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分散固相萃取-动态表面增强拉曼光谱法定量测定豆腐片中的金胺O

摘要 动态表面增强拉曼光谱(D-SERS)因其操作简单、灵敏度高而被广泛应用于化学分析中。开发了一种使用 D-SERS 定量测定豆腐片中金胺 O 的新方法。通过优化测量时间间隔和测定稳定性等SERS条件，在样品溶液蒸发过程中对金胺O进行了有效稳定的SERS测量。应用分散固相萃取以去除尽可能多的杂质。在对光谱进行评估之后，选择 SERS 光谱中 1114 cm-1 处的峰来量化豆腐片中存在的金胺 O。采用基质标准曲线-外标法进行统计分析。结果表明，在 0.5 至 10 mg/L 的范围内，色谱 SERS 峰与金胺 O 浓度之间具有良好的线性关系。相关系数 (R2) 大于 0.99。在 0.5、1.0 和 5.0 mg/kg 的加标水平下，豆腐片中金胺 O 的平均回收率为 92.3% 至 96%，相对标准偏差为 4.6% 至 8.3%。最低加标浓度被确定为定量限。因此，本研究为豆腐片中金胺O的定量测定提供了一种稳定、有效、快速的新方法。5.0 mg/kg，豆腐片中金胺O的平均回收率为92.3%~96%，相对标准偏差为4.6%~8.3%。最低加标浓度被确定为定量限。因此，本研究为豆腐片中金胺O的定量测定提供了一种稳定、有效、快速的新方法。5.0 mg/kg，豆腐片中金胺O的平均回收率为92.3%~96%，相对标准偏差为4.6%~8.3%。最低加标浓度被确定为定量限。因此，本研究为豆腐片中金胺O的定量测定提供了一种稳定、有效、快速的新方法。