We report on the development of high-throughput fluorogenic assay that can streamline directed evolution of enantioselective sulfoxide reductases. As a model, methionine sulfoxide reductase A (MsrA) has been evolved to expand its limited substrate scope. The resulting mutant MsrA can resolve a range of new challenging racemic sulfoxides with high efficiency including the pharmaceutically relevant albendazole sulfoxide. The simplicity and the level of throughput make this method also suitable for the screening of metagenomic libraries in future for the discovery of new enzymes with similar reactivities.

中文翻译：

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开发用于对映选择性亚砜还原酶的定向进化的简单高通量测定法。

我们报告了可以简化对映选择性亚砜还原酶的定向进化的高通量荧光检测的发展。作为模型，蛋氨酸亚砜还原酶A（MsrA）已得到发展，以扩大其有限的底物范围。所得的突变体MsrA可以高效分离一系列新的具有挑战性的外消旋亚砜，包括药学上相关的阿苯达唑亚砜。简便性和通量水平使该方法也适合将来筛选宏基因组文库，以发现具有类似反应性的新酶。