Accurate assessment of blood thrombosis and antithrombotic therapy is essential for the management of patients in a variety of clinical conditions, including surgery and on extracorporeal life support. However, current monitoring devices do not measure the effects of hemodynamic forces that contribute significantly to coagulation, platelet function and fibrin formation. This limits the extent to which current assays can predict clotting status in patients. Here, we demonstrate that a biomimetic microfluidic device consisting stenosed and tortuous arteriolar vessels would analyze blood clotting under flow, while requiring a small blood volume. When the device is connected to an inline pressure sensor a clotting time analysis is applied, allowing for the accurate measurement of coagulation, platelets and fibrin content. Furthermore, this device detects a prolonged clotting time in clinical blood samples drawn from pediatric patients on extracorporeal membrane oxygenation receiving anticoagulant therapy. Thus, this tortuosity activated microfluidic device could lead to a more quantitative and rapid assessment of clotting disorders and their treatment.

准确评估血栓形成和抗血栓形成疗法对于在各种临床条件下（包括手术和体外生命支持）管理患者至关重要。但是，当前的监测设备无法测量对凝血，血小板功能和血纤蛋白形成有重大贡献的血液动力的影响。这限制了当前的测定可以预测患者凝血状态的程度。在这里，我们证明了由狭窄的和弯曲的小动脉血管组成的仿生微流控设备将分析流动下的血液凝结，同时需要少量的血液。当设备连接到在线压力传感器时，将进行凝血时间分析，从而可以精确测量凝血，血小板和血纤蛋白的含量。此外，该设备可检测从接受抗凝治疗的体外膜氧合作用的儿科患者采集的临床血液样本中的凝血时间是否延长。因此，这种曲折性激活的微流体装置可以导致对凝血障碍及其治疗的更加定量和快速的评估。