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Eradication of Candida albicans persister cell biofilm by the membranotropic peptide gH625.
Scientific Reports ( IF 3.8 ) Pub Date : 2020-04-01 , DOI: 10.1038/s41598-020-62746-w
Emilia Galdiero 1 , Elisabetta de Alteriis 1 , Antonino De Natale 1 , Angela D'Alterio 1 , Antonietta Siciliano 1 , Marco Guida 1 , Lucia Lombardi 2 , Annarita Falanga 3 , Stefania Galdiero 2
Affiliation  

Biofilm formation poses an important clinical trouble due to resistance to antimicrobial agents; therefore, there is an urgent demand for new antibiofilm strategies that focus on the use of alternative compounds also in combination with conventional drugs. Drug-tolerant persisters are present in Candida albicans biofilms and are detected following treatment with high doses of amphotericin B. In this study, persisters were found in biofilms treated with amphotericin B of two clinical isolate strains, and were capable to form a new biofilm in situ. We investigated the possibility of eradicating persister-derived biofilms from these two Candida albicans strains, using the peptide gH625 analogue (gH625-M). Confocal microscopy studies allowed us to characterize the persister-derived biofilm and understand the mechanism of interaction of gH625-M with the biofilm. These findings confirm that persisters may be responsible for Candida biofilm survival, and prove that gH625-M was very effective in eradicating persister-derived biofilms both alone and in combination with conventional antifungals, mainly strengthening the antibiofilm activity of fluconazole and 5-flucytosine. Our strategy advances our insights into the development of effective antibiofilm therapeutic approaches.

中文翻译:


通过亲膜肽 gH625 根除白色念珠菌持久细胞生物膜。



由于对抗菌剂的耐药性,生物膜的形成带来了重要的临床问题;因此,迫切需要新的抗生物膜策略,重点关注替代化合物与常规药物的结合使用。白色念珠菌生物膜中存在耐药持久细胞,并在用高剂量两性霉素 B 处理后检测到。在这项研究中,在用两性霉素 B 处理的两种临床分离菌株的生物膜中发现了耐药持久细胞,并且能够在现场。我们研究了使用肽 gH625 类似物 (gH625-M) 根除这两种白色念珠菌菌株中源自持久性生物膜的可能性。共聚焦显微镜研究使我们能够表征持久性生物膜并了解 gH625-M 与生物膜相互作用的机制。这些研究结果证实,念珠菌生物膜的存活可能与念珠菌生物膜的存活有关,并证明gH625-M无论是单独使用还是与传统抗真菌药物联合使用,都能非常有效地根除念珠菌来源的生物膜,主要是增强氟康唑和5-氟胞嘧啶的抗生物膜活性。我们的策略推进了我们对开发有效抗生物膜治疗方法的见解。
更新日期:2020-04-01
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