ZZ-exchange spectroscopy is widely used to study slow exchange processes in biomolecules, especially determination of exchange rates and assignment of minor peaks. However, if the exchange cross peaks overlap or the populations are skewed, kinetic analysis is hindered. In order to analyze slow exchange protein dynamics under such conditions, here we have developed a new method by combining ZZ-exchange and F1F2-selective NMR spectroscopy. We demonstrate the utility of this method by examining the monomer-dimer transition of the ubiquitin-associated domain of p62, successfully assigning the minor (monomeric) peaks and obtaining the exchange rates, which cannot be achieved by ZZ-exchange alone.

中文翻译：

---

使用F1F2选择性ZZ交换光谱法对缓慢交换的蛋白质进行精确分析：分配和动力学分析。

ZZ交换光谱法广泛用于研究生物分子中的缓慢交换过程，尤其是确定交换速率和确定次要峰。但是，如果交换交叉峰重叠或总体偏斜，则会阻碍动力学分析。为了分析这种条件下的慢交换蛋白动力学，在这里我们结合了ZZ交换和F1F2选择性NMR光谱学开发了一种新方法。我们通过检查p62泛素相关结构域的单体-二聚体转变，成功分配次要（单体）峰并获得交换率来证明该方法的实用性，这仅凭ZZ交换是无法实现的。