In recent years, the use of microinjections has increased in life science and biotechnology fields; specific examples include artificial insemination and gene manipulation. Microinjections are mainly performed based on visual information; thus, the operator needs high-level skill because of the narrowness of the visual field. Additionally, microinjections are performed as the operator views a microscopic image on a display; the position of the display requires the operator to maintain an awkward posture throughout the procedure. In this study, we developed a microscopic image display apparatus for microinjections based on a view-expansive microscope. The prototype of the view-expansive microscope has problems related to the variations in brightness and focal blur that accompany changes in the optical path length and amount of reflected light. Therefore, we propose the use of a variable-focus device to expand the visual field and thus circumvent the above-mentioned problems. We evaluated the observable area of the system using this variable-focus device. We confirmed that the observable area is 261.4 and 13.9 times larger than that of a normal microscope and conventional view-expansive microscopic system, respectively. Finally, observations of mouse embryos were carried out by using the developed system. We confirmed that the microscopic images can be displayed on a head-mounted display in real time with the desired point and field sizes.

中文翻译：

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具有可自定义场大小和视点的基于头戴式显示器的显微成像系统。

近年来，在生命科学和生物技术领域中，显微注射的使用有所增加。具体例子包括人工授精和基因操纵。显微注射主要基于视觉信息进行；因此，由于视野狭窄，操作者需要高水平的技能。另外，当操作者在显示器上观看显微图像时，进行显微注射。显示器的位置要求操作员在整个过程中保持尴尬的姿势。在这项研究中，我们开发了一种基于视野扩展显微镜的显微注射用显微图像显示设备。视野扩大显微镜的原型存在与光程长度和反射光量的变化相关的亮度和焦点模糊的变化有关的问题。因此，我们建议使用可变焦装置来扩大视野，从而避免上述问题。我们使用这种变焦装置评估了系统的可观察区域。我们确认，可观察区域分别比普通显微镜和常规视野扩大显微镜系统大261.4和13.9倍。最后，使用开发的系统进行了小鼠胚胎的观察。我们确认可以将显微图像以所需的点和场大小实时显示在头戴式显示器上。我们确认，可观察区域分别比普通显微镜和常规视野扩大显微镜系统大261.4和13.9倍。最后，使用开发的系统进行了小鼠胚胎的观察。我们确认可以将显微图像以所需的点和场大小实时显示在头戴式显示器上。我们确认，可观察区域分别比普通显微镜和常规视野扩大显微镜系统大261.4和13.9倍。最后，使用开发的系统进行了小鼠胚胎的观察。我们确认可以将显微图像以所需的点和场大小实时显示在头戴式显示器上。