Society’s interest in the quality of food products with certain attributes has increased, the attribute of a Protected Designation of Origin (PDO) being an effective tool to guarantee the quality and geographical origin of a given food product. In Spain, two paprika production areas with PDO (La Vera and Murcia) are recognized. In the present work, targeted UHPLC-HRMS polyphenolic and capsaicinoid profiling through the TraceFinderTM screening software, using homemade accurate mass databases, was proposed as a source of chemical descriptors, to address the characterization, classification, and authentication of paprika. A total of 126 paprika samples from different production regions—Spain (La Vera PDO and Murcia PDO) and the Czech Republic, each including different flavor varieties, were analyzed. UHPLC-HRMS polyphenolic profiles showed to be good chemical descriptors to achieve paprika classification and authentication, based on the production region, through principal component analysis and partial least squares regression-discriminant analysis, with classification rates of 82%, 86%, and 100% for La Vera PDO, Murcia PDO, and the Czech Republic, respectively. In addition, a perfect classification was also accomplished among the flavor varieties for the Murcia PDO and Czech Republic samples. By employing the UHPLC-HRMS polyphenolic and capsaicinoid profiles as chemical descriptors, acceptable discrimination among La Vera PDO flavor varieties was also achieved.

中文翻译：

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靶向 UHPLC-HRMS (Orbitrap) 多酚和辣椒素分析，用于具有受保护原产地名称 (PDO) 属性的辣椒粉的化学计量学表征和分类

社会对具有某些属性的食品质量的兴趣已经增加，受保护的原产地名称 (PDO) 的属性是保证特定食品质量和地理来源的有效工具。在西班牙，有两个带有 PDO 的辣椒粉生产区（La Vera 和 Murcia）得到认可。在目前的工作中，建议使用自制的精确质量数据库通过 TraceFinderTM 筛选软件对 UHPLC-HRMS 多酚和辣椒素进行靶向分析，作为化学描述符的来源，以解决辣椒粉的表征、分类和认证问题。分析了来自不同产区——西班牙（La Vera PDO 和 Murcia PDO）和捷克共和国的 126 份辣椒粉样品，每个样品都包括不同的风味品种。通过主成分分析和偏最小二乘回归-判别分析，UHPLC-HRMS 多酚谱显示是实现辣椒粉分类和认证的良好化学描述符，分类率为 82%、86% 和 100%分别为 La Vera PDO、Murcia PDO 和捷克共和国。此外，穆尔西亚 PDO 和捷克共和国样品的风味品种也完成了完美的分类。通过使用 UHPLC-HRMS 多酚和辣椒素谱作为化学描述符，还实现了 La Vera PDO 风味品种之间可接受的区分。通过主成分分析和偏最小二乘回归-判别分析，La Vera PDO、Murcia PDO和捷克共和国的分类率分别为82%、86%和100%。此外，穆尔西亚 PDO 和捷克共和国样品的风味品种也完成了完美的分类。通过使用 UHPLC-HRMS 多酚和辣椒素谱作为化学描述符，还实现了 La Vera PDO 风味品种之间可接受的区分。通过主成分分析和偏最小二乘回归-判别分析，La Vera PDO、Murcia PDO和捷克共和国的分类率分别为82%、86%和100%。此外，穆尔西亚 PDO 和捷克共和国样品的风味品种也完成了完美的分类。通过使用 UHPLC-HRMS 多酚和辣椒素谱作为化学描述符，还实现了 La Vera PDO 风味品种之间可接受的区分。