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Recent development of an electrochemical imprinted sensor for the detection of trace-level of unmetabolized aflatoxin B2 in dairy milk
Journal of Electroanalytical Chemistry ( IF 4.1 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.jelechem.2020.114123
Nadia El Alami El Hassani , Benachir Bouchikhi , Nezha El Bari

Abstract Herein, we present and discuss the development of a novel sensor for aflatoxin B2 (AFB2) determination by two methods of functionalization. The first method was performed, by the electrodeposition of zinc oxide nanoparticles (ZnO-NPs), coated with a mixture of chitosan (CS) and AFB2 on the gold-working area of a screen-printed electrode. As for the second functionalization method, it was carried out by electropolymerization of pyrrole (Py) and AFB2 to form polypyrrole/AFB2 (PPy/AFB2) on another screen-printed electrode already modified with ZnO-NPs/CS composite. The morphology and the chemical composition of functionalized electrode surfaces were studied by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FT-IR). This system was successfully applied to determine AFB2 residues in fresh and pasteurized milk, with an excellent selectivity when compared to the results of interfering molecules. The limits of detection (LOD) and quantification (LOQ) were found to be 0.6 and 2.0 fg·mL−1 for ZnO-NPs/CS/AFB2 functionalized hybrid composite, and 0.2 and 0.7 fg·mL−1 for ZnO-NPs/CS/PPy/AFB2 functionalized hybrid composite when DPV measurements were used, respectively. The proposed technique is cheap, sensitive, selective, and is applied successfully to detect unmetabolized AFB2 in milk samples. Considering these advantages, our system provides an emerging approach application in industrial food control.

中文翻译:

用于检测牛奶中痕量未代谢黄曲霉毒素 B2 的电化学印迹传感器的最新进展

摘要 在此,我们介绍并讨论了通过两种功能化方法测定黄曲霉毒素 B2 (AFB2) 的新型传感器的开发。第一种方法是通过电沉积氧化锌纳米颗粒 (ZnO-NPs),在丝网印刷电极的金加工区域涂上壳聚糖 (CS) 和 AFB2 的混合物。至于第二种功能化方法,它是通过吡咯 (Py) 和 AFB2 的电聚合在另一个已经用 ZnO-NPs/CS 复合材料改性的丝网印刷电极上形成聚吡咯/AFB2 (PPy/AFB2) 来进行的。通过原子力显微镜 (AFM)、扫描电子显微镜 (SEM) 和傅里叶变换红外光谱 (FT-IR) 研究功能化电极表面的形态和化学成分。该系统成功应用于测定新鲜和巴氏杀菌牛奶中的 AFB2 残留,与干扰分子的结果相比具有出色的选择性。ZnO-NPs/CS/AFB2 功能化混合复合材料的检测限 (LOD) 和定量 (LOQ) 分别为 0.6 和 2.0 fg·mL-1,ZnO-NPs/当分别使用 DPV 测量时,CS/PPy/AFB2 功能化混合复合材料。所提出的技术廉价、灵敏、有选择性,并成功应用于检测牛奶样品中未代谢的 AFB2。考虑到这些优势,我们的系统在工业食品控制中提供了一种新兴的方法应用。ZnO-NPs/CS/AFB2 功能化混合复合材料的检测限 (LOD) 和定量 (LOQ) 分别为 0.6 和 2.0 fg·mL-1,ZnO-NPs/ 的检测限 (LOD) 和定量限 (LOQ) 分别为 0.2 和 0.7 fg·mL-1当分别使用 DPV 测量时,CS/PPy/AFB2 功能化混合复合材料。所提出的技术廉价、灵敏、有选择性,并成功应用于检测牛奶样品中未代谢的 AFB2。考虑到这些优势,我们的系统在工业食品控制中提供了一种新兴的方法应用。ZnO-NPs/CS/AFB2 功能化混合复合材料的检测限 (LOD) 和定量 (LOQ) 分别为 0.6 和 2.0 fg·mL-1,ZnO-NPs/ 的检测限 (LOD) 和定量限 (LOQ) 分别为 0.2 和 0.7 fg·mL-1当分别使用 DPV 测量时,CS/PPy/AFB2 功能化混合复合材料。所提出的技术便宜、灵敏、有选择性,并成功应用于检测牛奶样品中未代谢的 AFB2。考虑到这些优势,我们的系统在工业食品控制中提供了一种新兴的方法应用。
更新日期:2020-05-01
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