Abstract We report electrochemical response of non-adherent E. coli cells on a platinum disc electrode and in-situ inactivation through electro-stimulation to reduce their growth. Anodic peak current in cyclic voltammetry (CV) was gradually enhanced with an increased cell density due to increase in redox protein riboflavin secreted by the viable bacterial cells. The presence of the bacterial cells showed capacitive nature of the electric double layer capacitance and reduced interfacial charge transfer resistance in impedance of Nyquist plot. Formation of bacterial layer on the electrode surface was also evident from the rise in both anodic peak current and potential with scan rate at fixed cell concentration. The electrochemical inactivation of bacterial cells showed significant reduction in colony formation and growth. The observed inhibitory response with electro-stimulation was in comparison with the inactivation of bacterial cells treated with UV irradiation and sonication. Negative bias potential as compare to positive or periodic bias potential has been more effective for inactivation of the viable bacterial cells in suspension.

中文翻译：

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用于原位电化学灭活培养基中细菌生长的铂圆盘电极

摘要 我们报告了非粘附性大肠杆菌细胞在铂圆盘电极上的电化学响应和通过电刺激原位灭活以减少其生长。由于活细菌细胞分泌的氧化还原蛋白核黄素增加，循环伏安法 (CV) 中的阳极峰值电流随着细胞密度的增加而逐渐增强。细菌细胞的存在显示了双电层电容的电容性质和奈奎斯特图阻抗中降低的界面电荷转移电阻。从阳极峰值电流和电位随扫描速率在固定细胞浓度下的上升，电极表面上细菌层的形成也很明显。细菌细胞的电化学灭活显示集落形成和生长显着减少。观察到的电刺激抑制反应与用紫外线照射和超声处理的细菌细胞失活进行比较。与正偏压或周期性偏压相比，负偏压对于悬浮中的活细菌细胞的灭活更有效。