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Immune responses induced by inactivated vaccine against Aeromonas hydrophila in pacu, Piaractus mesopotamicus.
Fish & Shellfish Immunology ( IF 4.1 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.fsi.2020.03.059 Thais Heloisa Vaz Farias 1 , Salvador Arijo 2 , Alberto Medina 2 , Gabriela Pala 3 , Ed Jhonny da Rosa Prado 3 , Hélio José Montassier 4 , Fabiana Pilarski 1 , Marco Antonio de Andrade Belo 5
Fish & Shellfish Immunology ( IF 4.1 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.fsi.2020.03.059 Thais Heloisa Vaz Farias 1 , Salvador Arijo 2 , Alberto Medina 2 , Gabriela Pala 3 , Ed Jhonny da Rosa Prado 3 , Hélio José Montassier 4 , Fabiana Pilarski 1 , Marco Antonio de Andrade Belo 5
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Aeromonas hydrophila is responsible for outbreaks of a severe infectious disease in fish farms around the world and is one of the major causes of economic losses to the neotropical fish farmers. This study assessed the induction of immune responses and protection against A. hydrophila in pacu, Piaractus mesopotamicus, vaccinated through intraperitoneal and immersion route with inactivated virulent strain. Fish were randomly distributed in three vaccinated groups: intraperitoneal (i.p.) route; immersion; and immersion + booster; and control group (unvaccinated). All vaccination protocols used the concentration of 1.7 × 108 CFU mL-1 of inactivated A. hydrophila., and an oil adjuvant was used for vaccine prepararion for i.p. route vaccination. Blood and skin mucus from 9 fishes per treatment were collected at 14, 28, 42 and 84 days post-vaccination (DPV) for determination of lysozyme concentration in skin mucus, as well as antibodies anti-A. hydrophila in blood serum and skin mucus. Fish were challenged at 84 DPV with homologous and virulent strain of A. hydrophila for evaluation of resistance against bacterial infection. The results demonstrated that vaccination with inactivated A. hydrophila suspension by i.p. or immersion resulted in significant increase of skin mucus lysozyme and specific antibody levels in serum and skin mucus, at 28 and 42 DPV, and this increase in innate and adaptive immunity remained significant in pacu vaccinated through i.p. route up to 84 DPV. Although no significant differences were observed in the survival study, pacu vaccinated through i.p. route presented 31,33% of relative percentage survival (RPS) in LD50-96h when compared unvaccinated fish challenged at 84 DPV. The results observed in this study indicate that vaccination programs with inactivated A. hydrophila, including booster doses by i.p. or immersion routes, could result in more effective protection in pacu against this bacteriosis, by increasing innate and adaptive mucosal and systemic immune responses.
中文翻译：
灭活的疫苗对帕库（Piaractus mesopotamicus）中嗜水气单胞菌的灭活疫苗诱导。
嗜水气单胞菌是导致世界各地养鱼场爆发严重传染病的原因，也是导致新热带养鱼户经济损失的主要原因之一。这项研究评估了通过腹膜内和浸泡途径接种灭活的毒株后，在cucu，Piaractus mesopotamicus接种的cucu中，对免疫应答的诱导和对亲水曲霉的保护作用。将鱼随机分配到三个接种组中：腹膜内（ip）路线；浸没; 浸入式+助推器；对照组（未接种疫苗）。所有疫苗接种方案均使用浓度为1.7×108 CFU mL-1的灭活嗜水链球菌，并且将油佐剂用于ip途径疫苗的疫苗制备。每次处理时分别从14条，28条，9条鱼中采集血液和皮肤粘液，接种后42天和84天（DPV）测定皮肤粘液中的溶菌酶浓度，以及抗A抗体。血清和皮肤粘液中的亲水性。在84 DPV下用同源和强毒的嗜水链球菌菌株攻击鱼，以评估其对细菌感染的抵抗力。结果表明，通过腹膜内或浸入灭活的嗜水链球菌悬浮液进行接种可导致DPV在28和42时皮肤粘液溶菌酶和血清和皮肤粘液中的特异性抗体水平显着增加，而先天免疫和适应性免疫的这种增加在通过IP途径接种的pacu最高可达84 DPV。尽管在生存期研究中未观察到明显差异，但通过IP途径接种的pacu呈现31，与以84 DPV攻击的未接种鱼相比，LD50-96h中相对存活率（RPS）的33％。在这项研究中观察到的结果表明，通过增加先天性和适应性粘膜及全身免疫反应，对灭活的嗜水链球菌的疫苗接种计划（包括通过腹膜内或浸入途径的加强剂量）可以在帕库中更有效地预防这种细菌病。
更新日期：2020-04-01
中文翻译：
灭活的疫苗对帕库（Piaractus mesopotamicus）中嗜水气单胞菌的灭活疫苗诱导。
嗜水气单胞菌是导致世界各地养鱼场爆发严重传染病的原因，也是导致新热带养鱼户经济损失的主要原因之一。这项研究评估了通过腹膜内和浸泡途径接种灭活的毒株后，在cucu，Piaractus mesopotamicus接种的cucu中，对免疫应答的诱导和对亲水曲霉的保护作用。将鱼随机分配到三个接种组中：腹膜内（ip）路线；浸没; 浸入式+助推器；对照组（未接种疫苗）。所有疫苗接种方案均使用浓度为1.7×108 CFU mL-1的灭活嗜水链球菌，并且将油佐剂用于ip途径疫苗的疫苗制备。每次处理时分别从14条，28条，9条鱼中采集血液和皮肤粘液，接种后42天和84天（DPV）测定皮肤粘液中的溶菌酶浓度，以及抗A抗体。血清和皮肤粘液中的亲水性。在84 DPV下用同源和强毒的嗜水链球菌菌株攻击鱼，以评估其对细菌感染的抵抗力。结果表明，通过腹膜内或浸入灭活的嗜水链球菌悬浮液进行接种可导致DPV在28和42时皮肤粘液溶菌酶和血清和皮肤粘液中的特异性抗体水平显着增加，而先天免疫和适应性免疫的这种增加在通过IP途径接种的pacu最高可达84 DPV。尽管在生存期研究中未观察到明显差异，但通过IP途径接种的pacu呈现31，与以84 DPV攻击的未接种鱼相比，LD50-96h中相对存活率（RPS）的33％。在这项研究中观察到的结果表明，通过增加先天性和适应性粘膜及全身免疫反应，对灭活的嗜水链球菌的疫苗接种计划（包括通过腹膜内或浸入途径的加强剂量）可以在帕库中更有效地预防这种细菌病。
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