To study the effects of citric acid on fermentation process of Amomum villosum silage, A. villosum was ensiled without or with 1%, 2% citric acid and fermentation parameters and bacterial diversity were analyzed after 3, 7, 14, 30 days ensiling, respectively. Citric acid treated silages had lower dry matter loss (1.83% vs 2.23%), pH (3.84 vs 6.02), ammonia-N (0.33 vs 1.79 g/kg DM) and coliform bacteria number (<2.00 vs 7.27 log10 CFU/g FM) than the control after 30 days ensiling. The relative abundance of lactic acid bacteria, Pediococcus and Lactobacillus increased, whereas undesirable microorganisms like Enterobacter, Escherichia-Shigella and Pantoea decreased in citric acid treated A. villosum silage. These results indicated that quality A. villosum silage could be obtained by citric acid addition.

中文翻译：

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柠檬酸对砂仁青贮饲料发酵特性和细菌多样性的影响。

为了研究柠檬酸对砂仁青贮饲料发酵过程的影响，在不加或含1％，2％柠檬酸的条件下青贮砂仁，并分别在3、7、14、30天封盖后分析了发酵参数和细菌多样性。 。柠檬酸处理的青贮饲料具有较低的干物质损失（1.83％对2.23％），pH（3.84对6.02），氨氮（0.33对1.79 g / kg DM）和大肠菌群数量（<2.00对7.27 log10 CFU / g FM） ），比对30天后的对照。柠檬酸处理过的青霉菌青贮饲料中乳酸菌，小球菌和乳杆菌的相对丰度增加，而肠杆菌，大肠埃希菌-志贺氏菌和泛菌等不良微生物减少。这些结果表明，通过添加柠檬酸可以得到优质的沙枣青贮饲料。