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Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators.
Environmental Pollution ( IF 7.6 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.envpol.2020.114434 Julieta Peluso 1 , Carolina M Aronzon 1 , María Del Carmen Ríos de Molina 2 , Dante E Rojas 3 , Diego Cristos 3 , Cristina S Pérez Coll 1
Environmental Pollution ( IF 7.6 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.envpol.2020.114434 Julieta Peluso 1 , Carolina M Aronzon 1 , María Del Carmen Ríos de Molina 2 , Dante E Rojas 3 , Diego Cristos 3 , Cristina S Pérez Coll 1
Affiliation
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The Paraná River basin is one of the most important in South America and is affected by human activities that take place on its margins. In particular, the De la Cruz stream flows through an industrial pole and the Arrecifes River goes mainly through agricultural fields. The aim of this study was to evaluate the water quality of the De la Cruz stream (S1) and the Arrecifes River (S2) by means of physicochemical parameters, including metals and pesticides concentrations. Since amphibians are good indicators of environmental quality, bioassays with Rhinella arenarum were carried on. For lethal and sublethal parameters, embryos and larvae were exposed to a dilution gradient of water samples and AMPHITOX Solution (AS) as negative control for 504 h. For the determination of oxidative stress biomarkers (Catalase -CAT-, Glutathione S-Transferase -GST-, Reduced Glutathione -GSH-, and lipid peroxidation -TBARS-), embryos and larvae were exposed to undiluted water samples and AS. For the determination of micronuclei, larvae at hind limb bud stage (S.28) were exposed to undiluted water samples, simultaneously with negative and positive controls (AS and cyclophosphamide 40 mg/L, respectively). Dissolved oxygen was low in both sites and the copper levels exceeded the Argentine limit for the protection of aquatic life. In embryos exposure, water sample from S1 caused lethal effects (504h-LC50 = 49 (28-71.6)%), increased TBARS levels, and GST and CAT activities. In larvae exposure, water sample from this site decreased CAT activity, while the water sample from S2 caused important lethal effects (504h-LC50 = 98.72 (60.60-302.52)%), low GSH levels and increased GST activity. Water samples from both sites induced higher micronuclei frequency than the negative control. This study alerts about the degradation of water quality of the studied sites including lethal and sublethal effects in R. arenarum that can jeopardize the native populations of this species.
中文翻译：
通过理化和生物学指标对巴拉那河下游流域不同生产用途的水体质量进行综合分析。
巴拉那河流域是南美最重要的流域之一，受到其边缘人类活动的影响。特别是，德拉克鲁斯河流经工业极，而阿雷西费斯河则主要流经农田。这项研究的目的是通过物理化学参数，包括金属和农药浓度，评估德拉克鲁兹河（S1）和阿雷西费斯河（S2）的水质。由于两栖动物是环境质量的良好指标，因此进行了莱茵毛are的生物测定。对于致死参数和亚致死参数，将胚胎和幼虫暴露于水样品和AMPHITOX Solution（AS）的稀释梯度中，作为阴性对照，持续504 h。用于测定氧化应激生物标志物（过氧化氢酶-CAT-，谷胱甘肽S-转移酶-GST-，还原型谷胱甘肽-GSH-和脂质过氧化-TBARS-），胚胎和幼虫暴露于未稀释的水样品和AS中。为了测定微核，将后肢芽阶段的幼虫（S.28）暴露于未稀释的水样品中，同时与阴性和阳性对照（分别为AS和环磷酰胺40 mg / L）接触。两个站点的溶解氧都很低，并且铜的含量超过了阿根廷的极限，以保护水生生物。在胚胎暴露中，来自S1的水样品造成了致命的影响（504h-LC50 = 49（28-71.6）％），TBARS水平升高以及GST和CAT活性增加。在幼虫接触中，该部位的水样降低了CAT活性，而S2的水样引起了重要的致死作用（504h-LC50 = 98.72（60.60-302.52）％），低GSH含量和GST活性增加。来自两个位点的水样品诱导的微核频率均高于阴性对照。这项研究提示有关研究地点的水质下降，包括竞技场中的致死和亚致死作用，这可能会危害该物种的原生种群。
更新日期：2020-04-20
中文翻译：
通过理化和生物学指标对巴拉那河下游流域不同生产用途的水体质量进行综合分析。
巴拉那河流域是南美最重要的流域之一，受到其边缘人类活动的影响。特别是，德拉克鲁斯河流经工业极，而阿雷西费斯河则主要流经农田。这项研究的目的是通过物理化学参数，包括金属和农药浓度，评估德拉克鲁兹河（S1）和阿雷西费斯河（S2）的水质。由于两栖动物是环境质量的良好指标，因此进行了莱茵毛are的生物测定。对于致死参数和亚致死参数，将胚胎和幼虫暴露于水样品和AMPHITOX Solution（AS）的稀释梯度中，作为阴性对照，持续504 h。用于测定氧化应激生物标志物（过氧化氢酶-CAT-，谷胱甘肽S-转移酶-GST-，还原型谷胱甘肽-GSH-和脂质过氧化-TBARS-），胚胎和幼虫暴露于未稀释的水样品和AS中。为了测定微核，将后肢芽阶段的幼虫（S.28）暴露于未稀释的水样品中，同时与阴性和阳性对照（分别为AS和环磷酰胺40 mg / L）接触。两个站点的溶解氧都很低，并且铜的含量超过了阿根廷的极限，以保护水生生物。在胚胎暴露中，来自S1的水样品造成了致命的影响（504h-LC50 = 49（28-71.6）％），TBARS水平升高以及GST和CAT活性增加。在幼虫接触中，该部位的水样降低了CAT活性，而S2的水样引起了重要的致死作用（504h-LC50 = 98.72（60.60-302.52）％），低GSH含量和GST活性增加。来自两个位点的水样品诱导的微核频率均高于阴性对照。这项研究提示有关研究地点的水质下降，包括竞技场中的致死和亚致死作用，这可能会危害该物种的原生种群。
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