An analytical procedure using low-cost instrumentation (fluorescence/phosphorescence spectrophotometer) has been developed to assess total mercury in fishery products. Determinations were based on the room temperature phosphorescence (RTP) quenching of a composite Ph-QDs consisting of phenobarbital-containing polymer/silica coated Mn-doped ZnS quantum dots. Under optimum conditions (fish extract pH of 8.0, Ph-QDs concentration of 20 mg L⁻¹, and an interaction time of 12 min), the material offers high selectivity for inorganic mercury and methyl-mercury over other common ions present in the fish matrix. Moreover, good linearity was obtained for mercury concentrations within the 0–100 μg L⁻¹ range, and the obtained limit of detection (68.2 μg kg⁻¹) is low enough for a reliable assessment of total mercury in fish and seafood samples. The developed method was found to be free of matrix effects, and offers the advantage that the fish extracts can be directly analysed even at a 1:10 dilution. The method was found to be accurate after analysing a fish certified reference material, and after comparing total mercury levels in a set of fish samples analysed by the proposed chemosensor probe and by inductively coupled plasma mass spectrometry after an acid decomposition sample pre-treatment.

已经开发出一种使用低成本仪器（荧光/磷光分光光度计）的分析程序来评估渔业产品中的总汞。测定是基于复合Ph-QD的室温磷光（RTP）猝灭，该复合Ph-QD由含苯巴比妥的聚合物/二氧化硅包覆的Mn掺杂的ZnS量子点组成。在最佳条件下（鱼类提取物的pH值为8.0，Ph-QDs浓度为20 mg L ^-1，相互作用时间为12分钟），该材料对鱼类中存在的其他常见离子对无机汞和甲基汞的选择性高矩阵。此外，汞浓度在0–100μgL ^-1范围内具有良好的线性，并且检出限（68.2μgkg ^-1）足够低，无法可靠地评估鱼和海鲜样品中的总汞。发现开发的方法没有基质效应，并且具有即使以1:10的稀释度也可以直接分析鱼提取物的优点。在分析经过鱼类认证的参考材料之后，并且在对经过酸分解样品预处理的拟议化学传感器探针和电感耦合等离子体质谱法分析的一组鱼类样品中的总汞含量进行比较之后，发现该方法是准确的。