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Rapid determination of trace elements in serum of hepatocellular carcinoma patients by inductively coupled plasma tandem mass spectrometry
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.aca.2020.03.054 Liang Fu 1 , Hualin Xie 1 , Jianhua Huang 2 , Lin Chen 2
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.aca.2020.03.054 Liang Fu 1 , Hualin Xie 1 , Jianhua Huang 2 , Lin Chen 2
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The occurrence and development of hepatocellular carcinoma (HCC) is related to the level of trace elements. The mechanism of changes in trace element concentration and the identification of HCC risks associated with them are not clear. Therefore, an accurate and reliable approach for quantification of the level of trace elements in the serum of liver cancer patients is of great significance. In this study, we proposed a novel strategy for accurate determination of trace elements such as Mn, Fe, Ni, Cu, Zn, and Se in the serum of HCC patients based on inductively coupled plasma tandem mass spectrometry (ICP-MS/MS). In the MS/MS mode, N2O was used as the reaction gas, and an octopole reaction system (ORS) was employed to provide interference-free conditions and a sufficiently low limit of detection (LOD) for the determination of analytical elements. The internal standard elements with similar masses and similar mass spectrometry behavior were selected to correct the matrix effect. The LODs of Mn, Fe, Ni, Cu, Zn, and Se were as low as 0.013, 0.25, 0.010, 0.020, 0.17, and 0.008 μg L-1, respectively. Bovine serum (SRM 1598a) was adopted as the standard reference material to evaluate the accuracy of the method, and the developed method was successfully applied to determine the content of Mn, Fe, Cu, Zn, Ni, Se in serum from HCC patients. The obtained results were in agreement with that of sector field ICP-MS (SF-ICP-MS). The contents of Fe and Cu in the serum of HCC patients were higher compared to that of healthy controls, while the contents of Zn and Se were lower than that of healthy controls. Furthermore, no significant differences were observed in the contents of Mn and Ni in the serum of HCC patients and healthy controls.
中文翻译：
电感耦合等离子体串联质谱法快速测定肝癌患者血清中的微量元素
肝细胞癌（HCC）的发生发展与微量元素水平有关。微量元素浓度变化的机制以及与之相关的 HCC 风险的识别尚不清楚。因此，一种准确可靠的方法对肝癌患者血清中微量元素的含量进行定量具有重要意义。在本研究中，我们提出了一种基于电感耦合等离子体串联质谱 (ICP-MS/MS) 准确测定 HCC 患者血清中痕量元素如 Mn、Fe、Ni、Cu、Zn 和 Se 的新策略. 在 MS/MS 模式下，使用 N2O 作为反应气体，并采用八极杆反应系统 (ORS) 为分析元素的测定提供无干扰条件和足够低的检测限 (LOD)。选择具有相似质量和相似质谱行为的内标元素来校正基质效应。Mn、Fe、Ni、Cu、Zn 和 Se 的 LOD 分别低至 0.013、0.25、0.010、0.020、0.17 和 0.008 μg L-1。以牛血清（SRM 1598a）为标准参比物质，评价方法的准确性，所建立的方法成功用于测定HCC患者血清中Mn、Fe、Cu、Zn、Ni、Se的含量。所得结果与扇形场 ICP-MS (SF-ICP-MS) 的结果一致。HCC患者血清中Fe和Cu的含量高于健康对照组，而Zn和Se的含量低于健康对照组。此外，
更新日期：2020-05-01
中文翻译：
电感耦合等离子体串联质谱法快速测定肝癌患者血清中的微量元素
肝细胞癌（HCC）的发生发展与微量元素水平有关。微量元素浓度变化的机制以及与之相关的 HCC 风险的识别尚不清楚。因此，一种准确可靠的方法对肝癌患者血清中微量元素的含量进行定量具有重要意义。在本研究中，我们提出了一种基于电感耦合等离子体串联质谱 (ICP-MS/MS) 准确测定 HCC 患者血清中痕量元素如 Mn、Fe、Ni、Cu、Zn 和 Se 的新策略. 在 MS/MS 模式下，使用 N2O 作为反应气体，并采用八极杆反应系统 (ORS) 为分析元素的测定提供无干扰条件和足够低的检测限 (LOD)。选择具有相似质量和相似质谱行为的内标元素来校正基质效应。Mn、Fe、Ni、Cu、Zn 和 Se 的 LOD 分别低至 0.013、0.25、0.010、0.020、0.17 和 0.008 μg L-1。以牛血清（SRM 1598a）为标准参比物质，评价方法的准确性，所建立的方法成功用于测定HCC患者血清中Mn、Fe、Cu、Zn、Ni、Se的含量。所得结果与扇形场 ICP-MS (SF-ICP-MS) 的结果一致。HCC患者血清中Fe和Cu的含量高于健康对照组，而Zn和Se的含量低于健康对照组。此外，
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