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Integral characterization of normal and alopecic hair at different degeneration stages by in-situ visible and chemical imaging.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-03-31 , DOI: 10.1016/j.saa.2020.118315
Shi-Wei Hou 1 , Si-Yu He 2 , Jun Xie 2 , Ming-You Li 3 , Miao-Si Hong 4 , Fu-Lan Guan 5 , Yu-Ling Hu 6 , Yan-Li Huang 4 , Chang-Hua Xu 7
Affiliation  

Direct exploration to differences between normal hair (NH) and alopecic hair (AH) at different degeneration stages is still lacking. To reveal compositional and structural variation of AH with reference to NH internally and externally, infrared spectroscopic imaging combined with scanning electron microscopy was applied to investigate integral changes of hair chemical profiles and surface texture structures, and infrared macro-fingerprinting analysis revealed detailed chemical compositions of NH and AH. Results showed that AH had excessive irregular laminated structures compared to NH, leading to a lower weight bearing capacity. Spatial distributions of lipids, phosphates, lipoproteins and phospholipids in hair transverse sections showed that their infrared absorptions were intensified and gradually centralized to medulla with average variable-areas increasing upto 2.3 folds (lipoproteins area changed from 13% in NH to 30% in AH)as the alopecia progressed. Extracted pixel spectra from the chemical images showed different fingerprint characteristics in 1075-1120 cm-1. Specifically, compared to NH, AH showed red shift of phosphate peaks, indicating the occurrence of phosphates transformation. In this study, in-situ visible and infrared chemical imaging directly revealed more irregular laminated scalps with decreasing weight bearing capacity and increasing distributive areas expanding to medulla of key components (phosphates, phospholipids, etc.) that were relevant to alopecia development from NH to AH, and offered a fast, eco-friendly and effective method for hair research.

中文翻译:

通过原位可见光和化学成像对正常和秃发在不同变性阶段的整体表征。

仍然缺乏对不同变性阶段的正常头发(NH)和脱发(AH)之间差异的直接探索。为了从内部和外部揭示AH相对于NH的组成和结构变化,应用红外光谱成像结合扫描电子显微镜研究了头发化学轮廓和表面纹理结构的整体变化,红外宏观指纹分析揭示了AH的详细化学组成。 NH和AH。结果表明,与NH相比,AH具有过多的不规则叠层结构,导致较低的承重能力。脂质,磷酸盐,毛发横切面中的脂蛋白和磷脂显示,随着脱发的进行,它们的红外吸收增强并逐渐集中在髓质,平均可变区增加至2.3倍(脂蛋白面积从NH的13%变为AH的30%)。从化学图像中提取的像素光谱在1075-1120 cm-1处显示出不同的指纹特征。具体而言,与NH相比,AH显示出磷酸峰的红移,表明发生了磷酸盐转化。在这项研究中,原位可见光和红外化学成像直接显示出更多不规则的层状头皮,负重能力降低,分布区域扩展至延髓至关键成分(磷酸盐,磷脂等)的延髓,而这些成分与从脱发到脱发的过程有关。啊,
更新日期:2020-04-01
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