Abstract The genetic diversity by spa typing, the susceptibility to the sanitisers benzalkonium chloride (BC) and chlorhexidine (CHL) and the presence of efflux pump genes to resistance to sanitisers in 31 Staphylococcus aureus isolated from milk, were investigated. The spa type t127 was the most prevalent, which has a close genetic relationship with human isolates, and two new spa types (t18898 and t18899) were found in five isolates. Most of the evaluated isolates showed resistance to BC (90.3%), and all of them were resistant to CHL. The efflux pump genes mepA, norA and norB were more prevalent, detected in 77.4%, 80.6% and 100% of the isolates, respectively. Resistance to BC and CHL, and the presence of efflux pumps were observed in S. aureus isolates from milk, which are results reported for the first time in Brazil, emphasising the need for further studies in this field.

中文翻译：

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新的温泉类型、对消毒剂的抵抗力以及牛奶中金黄色葡萄球菌中外排泵基因的存在

摘要 研究了 31 株从牛奶中分离的金黄色葡萄球菌的 spa 分型的遗传多样性、对消毒剂苯扎氯铵 (BC) 和氯己定 (CHL) 的敏感性以及外排泵基因对消毒剂耐药性的存在。spa型t127是最普遍的，它与人类分离株有密切的遗传关系，在5个分离株中发现了两种新的spa型（t18898和t18899）。大多数被评估的分离株显示出对 BC 的抗性（90.3%），并且它们都对 CHL 具有抗性。外排泵基因 mepA、norA 和 norB 更为普遍，分别在 77.4%、80.6% 和 100% 的分离株中检测到。在从牛奶中分离出的金黄色葡萄球菌中观察到对 BC 和 CHL 的抗性以及外排泵的存在，这是巴西首次报道的结果，