Transforming petrochemical processes into bioprocesses has become an important goal of sustainable development. The chemical synthesis of 2,5‐furandicarboxylic acid (FDCA) from 5‐hydroxymethylfurfural (HMF) is expensive and environmentally unfavourable. The study aims to investigate a whole‐cell biocatalyst for efficient biotransformation of HMF to FDCA. For the first time, a genetically engineered Pseudomonas putida S12 strain expressing 5‐hydroxymethylfurfural oxidase (HMFO) was developed for the biocatalytic conversion of HMF to FDCA. This whole‐cell biocatalyst produced 35.7 mM FDCA from 50 mM HMF in 24 h without notable inhibition. However, when the initial HMF concentration was elevated to 100 mM, remarkable inhibition on FDCA production was observed, resulting in a reduction of FDCA yield to 42%. We solve this substrate inhibition difficulty by increasing the inoculum density. Subsequently, we used a fed‐batch strategy by maintaining low HMF concentration in the culture to maximize the final FDCA titre. Using this approach, 545 mM of FDCA was accumulatively produced after 72 hs, which is the highest production rate per unit mass of cells to the best of our knowledge.

中文翻译：

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通过恶臭假单胞菌 S12 中 5-羟甲基糠醛氧化酶的异质表达将 5-羟​​甲基糠醛绿色转化为呋喃-2,5-二甲酸。


将石化工艺转变为生物工艺已成为可持续发展的重要目标。从 5-羟甲基糠醛 (HMF) 化学合成 2,5-呋喃二甲酸 (FDCA) 成本昂贵且对环境不利。该研究旨在研究一种将 HMF 有效生物转化为 FDCA 的全细胞生物催化剂。首次开发了表达 5-羟甲基糠醛氧化酶 (HMFO) 的基因工程恶臭假单胞菌S12 菌株，用于 HMF 生物催化转化为 FDCA。这种全细胞生物催化剂在 24 小时内从 50 mM HMF 产生 35.7 mM FDCA，且没有明显的抑制作用。然而，当初始 HMF 浓度升高至 100 mM 时，观察到对 FDCA 产生的显着抑制，导致 FDCA 产量降低至 42%。我们通过增加接种密度解决了底物抑制困难。随后，我们使用补料分批策略，维持培养物中的低 HMF 浓度，以最大化最终 FDCA 滴度。使用这种方法，72小时后累计产生了545 mM的FDCA，这是据我们所知每单位质量细胞的最高生产率。