In horn flies, Haematobia irritans irritans (Diptera: Muscidae) (Linnaeus, 1758), target site resistance to pyrethroids can be diagnosed by an allele‐specific PCR that genotypes individual flies at both the super‐kdr (skdr) and the knock down resistance (kdr) associated loci. When this technique uses genomic DNA as template, modifications, such as alternative RNA splicing and RNA editing are not specifically detected. Alternative splicing at the skdr locus has been reported in Dipterans; thus, the genomic DNA‐based allele‐specific PCR may not accurately reflect the frequency of the skdr mutation in horn fly field populations. To investigate if alternative splicing occurs at the skdr locus of horn flies, genomic DNA and cDNA sequences isolated from two wild populations and two laboratory‐reared colonies with varying degrees of pyrethroid resistance were compared. There was no indication of alternative splicing at the super‐kdr locus neither in the wild populations nor in the laboratory‐reared colonies.

中文翻译：

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角蝇super-kdr基因座的序列和转录本表达

在角蝇中，血红素irritans irritans（Diptera：Muscidae）（Linnaeus，1758），对拟除虫菊酯的靶位点抗性可以通过等位基因特异性PCR诊断，该基因型在Super-kdr（skdr）和敲低抗性（kdr）相关位点均具有个体蝇的基因型。当此技术使用基因组DNA作为模板时，未特异检测到修饰，例如替代性RNA剪接和RNA编辑。在Dipterans中已经报道了在skdr基因座处的选择性剪接。因此，基于基因组DNA的等位基因特异性PCR可能无法准确反映角蝇场种群中skdr突变的频率。为了研究在角蝇的skdr位点是否发生其他剪接，比较了从两个野生种群和两个实验室饲养的不同拟除虫菊酯抗性菌落中分离的基因组DNA和cDNA序列。