当前位置: X-MOL 学术Biomater. Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
H2S-releasing amphiphilic dipeptide hydrogels are potent S. aureus biofilm disruptors.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-03-31 , DOI: 10.1039/d0bm00241k
Yun Qian 1 , Afnan Altamimi , Shaina Alston Yates , Santu Sarkar , Matthew Cochran , Mingjun Zhou , Nicole Levi-Polyachenko , John B Matson
Affiliation  

As a gasotransmitter, hydrogen sulfide (H2S) has been studied to treat wounds and inflammation, but its potential antimicrobial effects in this context have not been evaluated. An H2S-releasing dipeptide hydrogel (S-FE), and several non-H2S-releasing control dipeptides, (C-FE, C-GE, FBA-FE, and FE where S = S-aroylthiooxime, an H2S donor; C = control, an oxime incapable of H2S release; FBA = 4-formylbenzamide, also incapable of H2S release; and E, F, G = glutamic acid, phenylalanine, and glycine, respectively), were studied to correlate differences in their chemical structures and H2S-releasing abilities with their antimicrobial effects on Staphylococcus aureus bacteria. Dipeptides with Phe (S-FE, C-FE, and FE) self-assembled into nanoribbons in water and displayed β-sheet formation and enhanced fluorescence, while the other two dipeptides (FBA-FE and C-GE) did not form assemblies in water. In vitro experiments with Staphylococcus aureus, which is a commonly found bacterium associated with wounds, showed significant antimicrobial effects from some of the dipeptides. Dipeptide S-FE inhibited bacterial growth more effectively than any of the controls, thereby limiting biofilm formation or disrupting established biofilms. These antimicrobial H2S-releasing dipeptide hydrogels provide a promising new approach to treat wound infections.

中文翻译:


释放 H2S 的两亲性二肽水凝胶是有效的金黄色葡萄球菌生物膜破坏剂。



作为一种气体递质,硫化氢 (H2S) 已被研究用于治疗伤口和炎症,但其在这方面的潜在抗菌作用尚未得到评估。释放 H2S 的二肽水凝胶 (S-FE) 和几种不释放 H2S 的对照二肽(C-FE、C-GE、FBA-FE 和 FE,其中 S = S-芳酰硫肟,H2S 供体;C =对照,不能释放 H2S 的肟;FBA = 4-甲酰基苯甲酰胺,也不能释放 H2S;E、F、G 分别 = 谷氨酸、苯丙氨酸和甘氨酸),研究了它们的化学结构和 H2S 的差异。 -释放对金黄色葡萄球菌的抗菌作用。含有 Phe 的二肽(S-FE、C-FE 和 FE)在水中自组装成纳米带,并表现出 β-折叠形成和增强的荧光,而其他两种二肽(FBA-FE 和 C-GE)没有形成组装体在水中。金黄色葡萄球菌(一种常见的伤口相关细菌)的体外实验显示,某些二肽具有显着的抗菌作用。二肽 S-FE 比任何对照物更有效地抑制细菌生长,从而限制生物膜形成或破坏已建立的生物膜。这些释放 H2S 的抗菌二肽水凝胶为治疗伤口感染提供了一种有前景的新方法。
更新日期:2020-03-31
down
wechat
bug