GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.

中文翻译：

---

用于社交变形虫的合成生物学的GoldenBraid克隆系统。

GoldenBraid是一个快速，模块化且功能强大的克隆系统，用于组装和组合遗传元件。变形杆菌属（Dictyostelium amoebae）代表了一种有趣的合成生物底物，在开发，趋化性，细菌-宿主相互作用和同种异体认知方面具有广泛应用。我们目前将GoldenBraid作为Dictyostelium的合成生物学框架，包括一个包含250个DNA零件和组件的文库以及一个概念证明菌株，该菌株说明了cAMP趋化性，其中四个荧光报告基因由一个质粒编码。