We used a multi-omics profiling approach to investigate the suppressive effects of 2% Wolfberry (WOL)-enriched diets on dextran sodium sulfate (DSS)-induced colitis in mice. It was observed that in mice fed the WOL diet, the disease activity index, colon shortening, plasma concentrations of matrix metalloproteinase-3 and relative mesenteric fat weight were significantly improved as compared to the DSS group. Results from colon transcriptome and proteome profiles showed that WOL supplementation significantly ameliorated the expression of genes and proteins associated with the integrity of the colonic mucosal wall and colonic inflammation. Based on the hepatic transcriptome, proteome and metabolome data, genes involved in fatty acid metabolism, proteins involved in inflammation and metabolites related to glycolysis were downregulated in WOL mice, leading to lowered inflammation and changes in these molecules may have led to improvement in body weight loss. The integrated nutrigenomic approach thus revealed the molecular mechanisms underlying the ameliorative effect of whole WOL fruit consumption on inflammatory bowel disease.

我们使用了一种多组学分析方法来研究富含2％枸杞（WOL）的饮食对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的抑制作用。观察到，与DSS组相比，在以WOL饮食喂养的小鼠中，疾病活动指数，结肠缩短，基质金属蛋白酶3血浆浓度和肠系膜脂肪重量显着改善。结肠转录组和蛋白质组图谱的结果表明，补充WOL可以显着改善与结肠粘膜壁完整性和结肠炎症相关的基因和蛋白质的表达。根据肝转录组，蛋白质组和代谢组数据，在WOL小鼠中，与脂肪酸代谢有关的基因，与炎症有关的蛋白质和与糖酵解有关的代谢产物被下调，导致炎症降低，这些分子的变化可能导致体重减轻。因此，综合营养学方法揭示了食用整个WOL水果对炎症性肠病改善作用的分子机制。