PURPOSE Immunotherapy has gained traction in the treatment of solid tumors but the immunological landscape of pituitary adenomas is not well defined. We sought to investigate the immunological composition in pituitary adenomas using RNA deconvolution (CIBERSORTx) on an existing gene expression dataset for pituitary adenomas. METHODS We applied an established computational approach (CIBERSORTx) on 134 pituitary adenomas from a previously published gene expression dataset to infer the proportions of 22 subsets of immune cells. We investigated associations between each immune cell type and tumor subtype. RESULTS We found that the majority of infiltrating immune cells within pituitary adenomas were comprised of M2 macrophages followed by resting CD4+ memory T cells and mast cells. Silent pituitary tumors have higher M2 macrophage fractions when compared to other subtypes. In contrast, Cushing pituitary tumors, both overt and subclinical cases, had higher CD8+ T cells fractions than GH tumors, prolactinomas, hyperthyroid tumors, and silent tumors. CONCLUSIONS RNA deconvolution of the immune infiltrates of pituitary adenomas using CIBERSORTx suggests that most pituitary adenomas comprise of M2 macrophages, but each adenoma subtype has a unique immune landscape. This may have implications in targeting each adenoma subtype with different immunotherapies.

中文翻译：

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垂体腺瘤免疫学特征的计算机模拟分析。

目的免疫疗法在实体瘤的治疗中获得了广泛的关注，但垂体腺瘤的免疫学前景尚不明确。我们试图在垂体腺瘤的现有基因表达数据集上使用 RNA 解卷积 (CIBERSORTx) 研究垂体腺瘤的免疫学组成。方法我们对来自先前公布的基因表达数据集的 134 个垂体腺瘤应用了一种既定的计算方法 (CIBERSORTx)，以推断 22 个免疫细胞亚群的比例。我们研究了每种免疫细胞类型与肿瘤亚型之间的关联。结果我们发现垂体腺瘤内的大部分浸润性免疫细胞由 M2 巨噬细胞组成，其次是静息 CD4+ 记忆 T 细胞和肥大细胞。与其他亚型相比，无症状垂体瘤具有更高的 M2 巨噬细胞分数。相比之下，库欣垂体瘤，无论是明显的还是亚临床病例，其 CD8+ T 细胞分数都高于 GH 肿瘤、催乳素瘤、甲状腺功能亢进肿瘤和无症状肿瘤。结论 使用 CIBERSORTx 对垂体腺瘤免疫浸润的 RNA 解卷积表明，大多数垂体腺瘤由 M2 巨噬细胞组成，但每种腺瘤亚型都有独特的免疫景观。这可能对用不同的免疫疗法靶向每种腺瘤亚型有影响。结论 使用 CIBERSORTx 对垂体腺瘤免疫浸润的 RNA 解卷积表明，大多数垂体腺瘤由 M2 巨噬细胞组成，但每种腺瘤亚型都有独特的免疫景观。这可能对用不同的免疫疗法靶向每种腺瘤亚型有影响。结论 使用 CIBERSORTx 对垂体腺瘤免疫浸润的 RNA 解卷积表明，大多数垂体腺瘤由 M2 巨噬细胞组成，但每种腺瘤亚型都有独特的免疫景观。这可能对用不同的免疫疗法靶向每种腺瘤亚型有影响。