Abstract

The potential of lysozyme to degrade bacterial cell wall has found important applications in the fields of medicine and biotechnology. The ability of methylene blue dye to bind to the cell walls of bacteria was utilized for development of an assay method for measurement of lysozyme activity. The method was calibrated with the commercial preparation of lysozyme using Micrococcus lysodeikticus cells as substrate. The method was functional over a range of 5–40 U/mL. The method was compared with the conventional turbidimetric method. Sensitivity of the method was similar to turbidimetric method as both the methods could detect lysozyme activity as low as 5 U/mL. Enzyme activity in saliva samples measured by both the methods was comparable and ranged from 1000 to 2100 U/mL.

中文翻译：

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亚甲基蓝作为细胞壁结合剂的溶菌酶比色法的开发

摘要

已经发现溶菌酶降解细菌细胞壁的潜力在医学和生物技术领域中具有重要的应用。利用亚甲基蓝染料结合细菌细胞壁的能力来开发用于测定溶菌酶活性的测定方法。使用溶菌微球菌细胞作为底物，用商业溶菌酶制剂对方法进行校准。该方法在5–40 U / mL范围内起作用。将该方法与常规比浊法进行了比较。该方法的灵敏度与比浊法相似，因为这两种方法均可检测到低至5 U / mL的溶菌酶活性。通过这两种方法测量的唾液样品中的酶活性相当，范围为1000至2100 U / mL。