This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.

中文翻译：

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从美国东部得克萨斯州收集的蚊子中分离出一种新型玻虫病毒（Birnaviridae）的波利瓦尔病毒的表征。

这份报告描述并描述了一种新型昆虫性鼻病毒，称为波利瓦尔港病毒（PTBV），该病毒是从美国东德克萨斯州的一个海水沼泽中收集的伊蚊中分离出来的。全基因组测序和系统发育分析表明，PTBV是独特的，但与果蝇X病毒和蚊子X病毒具有遗传相关性，而果蝇X病毒和蚊子X病毒被归类为昆虫疫病毒属Birnaviridae属。PTBV在蚊子（C6 / 36）细胞培养物中产生了细胞病变效应（CPE），但在Vero细胞培养物中却没有。在感染的C6 / 36细胞中PTBV的超微结构研究表明，直径约55 nm的未包被病毒颗粒。