All known polymerases copy genetic material by catalyzing phosphodiester bond formation. This highly conserved activity proceeds by a common mechanism, such that incorporated nucleoside analogs terminate chain elongation if the resulting primer strand lacks a terminal hydroxyl group. Even conservatively substituted 3′-amino nucleotides generally act as chain terminators, and no...

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修饰的DNA聚合酶合成氨基磷酸酯连接的DNA [化学]

所有已知的聚合酶都通过催化磷酸二酯键的形成来复制遗传物质。这种高度保守的活性通过共同机制进行，使得如果所得的引物链缺少末端羟基，则掺入的核苷类似物终止链延长。即使是保守取代的3'-氨基核苷酸也通常充当链终止剂，并且没有...