Abstract In this work, gelatin-g-poly(acrylic acid) based pH-sensitive nanocomposite hydrogels incorporated with Layered Double Hydroxide (LDH) were developed. Using various spectroscopic techniques such as FTIR, XRD, SEM, EDX and TEM, the polymeric hydrogels were characterized. FTIR confirmed grafting of partially neutralized Acrylic Acid (AAc) on the backbone of Gelatin. Incorporation of LDH fillers inside the hydrogel matrix was established from XRD data. Inclusion of LDH resulted wrinkled morphology of the surface of the hydrogel from the SEM images. Formation of nanocomposites were further evaluated by TEM analysis which displayed significant change in the TEM morphology of the nanocomposite hydrogel. Using DLS, particle size for LDH were determined further and was found to be around 100 nm. Swelling value of the hydrogel was around 34 g/g. Biocompatibility results showed enhanced blood compatibility for the hydrogels. The controlled release studies performed using the hydrogels demonstrated vitamin B12 was released in Artificial Gastric Fluid (AGF) and in Artificial Intestinal Fluid (AIF) in a controlled manner. The release study was carried out for 7 h each and was evaluated using UV–visible spectrophotometer. From the curves, the cumulative release in AGF and AIF was found to be 24% and 31%, respectively.

中文翻译：

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丙烯酸接枝明胶/LDH基生物相容性水凝胶，具有pH可控释放维生素B12

摘要 在这项工作中，开发了基于明胶-g-聚（丙烯酸）的 pH 敏感纳米复合水凝胶，并掺入了层状双氢氧化物（LDH）。使用各种光谱技术，如 FTIR、XRD、SEM、EDX 和 TEM，对聚合物水凝胶进行了表征。FTIR 证实了部分中和的丙烯酸 (AAc) 在明胶骨架上的接枝。根据 XRD 数据确定了 LDH 填料在水凝胶基质中的掺入。从 SEM 图像中包含 LDH 导致水凝胶表面的皱纹形态。通过TEM分析进一步评估纳米复合材料的形成，其显示纳米复合材料水凝胶的TEM形态发生显着变化。使用 DLS，进一步确定 LDH 的粒径，发现约为 100 nm。水凝胶的溶胀值约为 34 g/g。生物相容性结果显示水凝胶的血液相容性增强。使用水凝胶进行的控释研究表明，维生素 B12 在人工胃液 (AGF) 和人工肠液 (AIF) 中以受控方式释放。释放研究每次进行 7 小时，并使用紫外-可见分光光度计进行评估。从曲线中，发现 AGF 和 AIF 的累积释放量分别为 24% 和 31%。