A new bioprocess has been developed that allows for producing β-carotene from the xylose portion of bioenergy sorghum. Bioenergy sorghum was pretreated in a pilot-scale continuous hydrothermal reactor followed by disc refining. Xylose was extracted using low-severity dilute acid hydrolysis. A xylose yield of 64.9% (17.4 g/L) was obtained by hydrolyzing at 120 °C for 5 min with 2% sulfuric acid. The xylose-enriched syrup was separated and concentrated to either 32 g xylose/L (medium-concentrated hydrolysate, MCB) or 66 g xylose/L (high-concentrated hydrolysate, HCB). The non- (NCB), medium-, and high-concentrated xylose syrup were neutralized and fermented to β-carotene using Saccharomyces cerevisiae strain SR8B, which had been engineered for xylose utilization and β-carotene production. In HCB, MCB, and NCB cultures, the yeast produced β-carotene titers of 114.50 mg/L, 93.56 mg/L, and 82.50 mg/L, which corresponds to specific yeast biomass productions of 7.32 mg/g DCW, 8.10 mg/g DCW, and 8.29 mg/g DCW, respectively.

中文翻译：

---

从生物能源高粱生产富含木糖的水解产物，并使用工程酿酒酵母将其转化为β-胡萝卜素。

已经开发出一种新的生物工艺，该工艺可以从生物能高粱的木糖部分生产β-胡萝卜素。生物能源高粱在中试规模的连续水热反应器中进行预处理，然后进行圆盘精制。使用低强度稀酸水解提取木糖。通过在120°C下用2％的硫酸水解5分钟，木糖收率为64.9％（17.4 g / L）。分离富含木糖的糖浆并浓缩至32 g木糖/ L（中等浓度的水解物，MCB）或66 g木糖/ L（高浓度水解物，HCB）。使用酿酒酵母菌株SR8B中和非（NCB），中浓度和高浓度的木糖糖浆，并将其发酵为β-胡萝卜素。在HCB，MCB和NCB文化中，