A porous polymer membrane-based d-amino acid oxidase (DAAO) reactor was developed that mimicked enzymatic activity in a renal ischemia model. Using glycidyl methacrylate as a biocompatible reactive monomer, poly(styrene-glycidyl methacrylate) was synthesized via a reversible addition fragment chain transfer polymerization technique. The prepared porous polymer membrane was used as a support to effectively immobilize DAAO. Compared to DAAO modified on nonporous polymer membrane and free DAAO in solution, the constructed porous polymer membrane-based DAAO enzyme reactor displayed 3-fold and 19-fold increase in enzymolysis efficiency, respectively. In addition, a chiral ligand exchange capillary electrophoresis system for DAAO was used to study DAAO enzymatic kinetics with d,l-methionine as the substrate. The proposed porous polymer membrane-based enzyme reactor showed excellent performance both on reproducibility and stability. Moreover, the enzyme reactor was successfully applied to mimic DAAO activity in a renal ischemia model. These results demonstrated that the enzyme could be efficiently immobilized onto a porous polymer membrane as an enzyme reactor and has great potential in mimicking the enzymatic activity in kidney.

开发了一种基于多孔聚合物膜的d-氨基酸氧化酶（DAAO）反应器，该反应器模仿了肾脏缺血模型中的酶活性。使用甲基丙烯酸缩水甘油酯作为生物相容性反应性单体，通过可逆的加成片段链转移聚合技术合成了聚（甲基丙烯酸苯乙烯-缩水甘油酯）。所制备的多孔聚合物膜用作支持物以有效地固定DAAO。与在无孔聚合物膜上修饰的DAAO和溶液中的游离DAAO相比，构建的基于多孔聚合物膜的DAAO酶反应器的酶解效率分别提高了3倍和19倍。此外，对于DAAO手性配体交换毛细管电泳系统被用来研究DAAO具有酶促动力学d，升-甲硫氨酸作为基材。提出的基于多孔聚合物膜的酶反应器在重现性和稳定性方面均显示出优异的性能。此外，该酶反应器已成功应用于模拟肾缺血模型中的DAAO活性。这些结果表明，该酶可以有效地固定在作为酶反应器的多孔聚合物膜上，并且在模仿肾脏中的酶活性方面具有很大的潜力。