A fast off-line FPSE-HPLC-PDA method has been reported that allows simultaneous clean up and determination of six non-steroidal anti-inflammatory drugs (NSAIDs) in saliva samples from healthy volunteers. Particularly, furprofen, indoprofen, ketoprofen, fenbufen, flurbiprofen, and ibuprofen were chromatographically resolved. Benzyl paraben was chosen as the internal standard (BzPB, IS). These target compounds were successfully extracted from human saliva using fabric phase sorptive extraction (FPSE) and then analysed in the liquid chromatographic system by means of a short analytical column (Symmetry C18, 75 × 4.6 mm, 3.5 µm) using acetonitrile (AcN) and phosphate buffer (PBS, 30 mM; pH = 2.5) as the mobile phases. The method, validated through the calculation of all analytical parameters in accordance of International Guidelines, was applied to real saliva sample analysis collected from informed volunteers. The proposed approach that included the use of sol-gel polytetrahydrofuran (sol-gel PTHF) sorbent immobilized on cellulose support and C18 stationary phase used in HPLC, showed high potential as a fast tool for future clinical and forensic applications. The herein reported results encourage potential future application of FPSE in the forensic field. Furthermore, the FPSE membrane was tested in dried saliva spot mode (DSS) in order to check its potential use as a sampling device, also for forensic applications.

中文翻译：

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快速离线FPSE-HPLC-PDA测定唾液样品中的6种NSAID。

据报道，一种快速离线的FPSE-HPLC-PDA方法可以同时清除和测定健康志愿者唾液样品中的六种非甾体类抗炎药（NSAID）。特别地，将呋喃芬，吲哚洛芬，酮洛芬，芬布芬，氟比洛芬和布洛芬色谱分离。选择对羟基苯甲酸苄酯作为内标（BzPB，IS）。使用织物相吸附萃取（FPSE）成功地从人唾液中提取了这些目标化合物，然后在液相色谱系统中通过使用乙腈（AcN）的短分析柱（对称性C18，75×4.6 mm，3.5 µm）进行分析。磷酸盐缓冲液（PBS，30 mM； pH = 2.5）作为流动相。该方法通过根据国际准则计算所有分析参数而得到验证，应用于从知情志愿者那里收集的真实唾液样本分析。所提议的方法包括使用固定在纤维素支持物上的溶胶-凝胶聚四氢呋喃（sol-gel PTHF）吸附剂和HPLC中使用的C18固定相，显示出作为将来临床和法医学应用的快速工具的巨大潜力。本文报道的结果鼓励FPSE在法医领域的潜在未来应用。此外，以干唾液斑点模式（DSS）对FPSE膜进行了测试，以检查其作为采样设备的潜在用途，也可用于法医应用。具有很高的潜力，可以作为未来临床和法医应用的快速工具。本文报道的结果鼓励FPSE在法医领域的潜在未来应用。此外，以干唾液斑点模式（DSS）对FPSE膜进行了测试，以检查其作为采样设备的潜在用途，也可用于法医应用。具有很高的潜力，可以作为未来临床和法医应用的快速工具。本文报道的结果鼓励FPSE在法医领域的潜在未来应用。此外，以干唾液斑点模式（DSS）对FPSE膜进行了测试，以检查其作为采样设备的潜在用途，也可用于法医应用。