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D-optimal design as a useful tool response surface methodology for the optimization of signals from synchronous fluorescence prior to simultaneous determination of avanafil and tadalafil.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-03-31 , DOI: 10.1016/j.saa.2020.118313 Ahmed M Abdel-Raoof 1 , Ragab A M Said 2 , Mohamed S Emara 1 , Ebrahim A El-Desouky 1 , Ahmed M Abdelzaher 1 , Mohamed A Hasan 1 , Ayman E Osman 1
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-03-31 , DOI: 10.1016/j.saa.2020.118313 Ahmed M Abdel-Raoof 1 , Ragab A M Said 2 , Mohamed S Emara 1 , Ebrahim A El-Desouky 1 , Ahmed M Abdelzaher 1 , Mohamed A Hasan 1 , Ayman E Osman 1
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A rapid, smart and sensitive first derivative spectrofluorimetric method has been carried out for the simultaneous estimation of avanafil and tadalafil either in their pure form, tablet dosage form or spiked human plasma. The measurements of normal emission spectra or synchronous fluorescence intensity of both drugs show severe overlap which hindered their determination using normal fluorescence or synchronous intensity. Therefore, a highly sensitive first derivative synchronous fluorescence procedure was used to resolve this overlap. The method is based upon measurement of the amplitude of the first derivative of synchronous fluorescence intensity of both drugs at Δλ = 70 nm and at suitable wavelength of 396 nm and 364 nm for avanafil and tadalafil, respectively. Under the optimum conditions, the linear determination ranges are 50-1800 and 5-400 ng mL-1 with a detection limit of 12.93 and 1.46 ng mL-1 for avanafil and tadalafil, respectively. A response surface methodology was used for optimization using D-optimal design which can be used for determination of the exact optimum parameters specifically designed for this method. In addition; it is a good way to graphically clarify the relationship between various experimental variables and the synchronous fluorescence intensity.
中文翻译：
D最佳设计是一种有用的工具响应表面方法，用于在同时测定avanafil和tadalafil之前优化同步荧光信号。
已经进行了快速，智能和灵敏的一阶导数荧光光谱法，用于同时估算纯形式，片剂剂型或加标人体血浆中的阿伐那非和他达拉非。两种药物的正常发射光谱或同步荧光强度的测量结果显示出严重的重叠，这妨碍了它们使用正常荧光或同步强度进行测定。因此，使用高度敏感的一阶导数同步荧光程序来解决此重叠问题。该方法基于两种药物的同步荧光强度的一阶导数在Δλ= 70 nm以及适当的avanafil和tadalafil的396 nm和364 nm波长处的测量。在最佳条件下 线性测定范围为50-1800和5-400 ng mL-1，阿伐那非和他达拉非的检出限分别为12.93和1.46 ng mL-1。响应面方法用于D-最优设计的优化，可用于确定专门为此方法设计的确切最优参数。此外; 这是一种以图形方式阐明各种实验变量与同步荧光强度之间关系的好方法。
更新日期：2020-03-31
中文翻译：
D最佳设计是一种有用的工具响应表面方法，用于在同时测定avanafil和tadalafil之前优化同步荧光信号。
已经进行了快速，智能和灵敏的一阶导数荧光光谱法，用于同时估算纯形式，片剂剂型或加标人体血浆中的阿伐那非和他达拉非。两种药物的正常发射光谱或同步荧光强度的测量结果显示出严重的重叠，这妨碍了它们使用正常荧光或同步强度进行测定。因此，使用高度敏感的一阶导数同步荧光程序来解决此重叠问题。该方法基于两种药物的同步荧光强度的一阶导数在Δλ= 70 nm以及适当的avanafil和tadalafil的396 nm和364 nm波长处的测量。在最佳条件下 线性测定范围为50-1800和5-400 ng mL-1，阿伐那非和他达拉非的检出限分别为12.93和1.46 ng mL-1。响应面方法用于D-最优设计的优化，可用于确定专门为此方法设计的确切最优参数。此外; 这是一种以图形方式阐明各种实验变量与同步荧光强度之间关系的好方法。
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