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Electronic cigarette exposure disrupts blood-brain barrier integrity and promotes neuroinflammation
Brain, Behavior, and Immunity ( IF 8.8 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.bbi.2020.03.034 Nathan A Heldt 1 , Alecia Seliga 2 , Malika Winfield 2 , Sachin Gajghate 2 , Nancy Reichenbach 2 , Xiang Yu 3 , Slava Rom 1 , Amogha Tenneti 2 , Dana May 2 , Brian D Gregory 3 , Yuri Persidsky 1
Brain, Behavior, and Immunity ( IF 8.8 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.bbi.2020.03.034 Nathan A Heldt 1 , Alecia Seliga 2 , Malika Winfield 2 , Sachin Gajghate 2 , Nancy Reichenbach 2 , Xiang Yu 3 , Slava Rom 1 , Amogha Tenneti 2 , Dana May 2 , Brian D Gregory 3 , Yuri Persidsky 1
Affiliation
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Electronic cigarette (e-cigarette) use has grown substantially since inception, particularly among adolescents and combustible tobacco users. Several cigarette smoke constituents with known neurovascular effect are present in e-cigarette liquids or formed during the vapor generation. The present study establishes inhaled models of cigarette and e-cigarette use with normalized nicotine delivery, then characterizes the impact on blood-brain barrier function. Sequencing of microvessel RNA following exposure revealed downregulation of several genes with critical roles in BBB function. Reduced protein expression of Occludin and Glut1 is also observed at the tight junction in all groups following exposure. Pro-inflammatory changes in leukocyte-endothelial cell interaction are also noted, and mice exposed to nicotine-free e-cigarettes have impaired novel object recognition performance. On this basis, it is concluded that long term e-cigarette use may adversely impact neurovascular health. The observed effects are noted to be partly independent of nicotine content and nicotine may even serve to moderate the effects of non-nicotinic components on the blood-brain barrier.
中文翻译：
接触电子烟会破坏血脑屏障的完整性并促进神经炎症
自诞生以来，电子烟（e-香烟）的使用量大幅增长，尤其是在青少年和可燃烟草使用者中。几种具有已知神经血管作用的香烟烟雾成分存在于电子烟液体中或在蒸汽产生过程中形成。本研究建立了具有标准化尼古丁递送的香烟和电子烟吸入模型，然后表征对血脑屏障功能的影响。暴露后微血管 RNA 的测序揭示了在 BBB 功能中具有关键作用的几个基因的下调。在暴露后所有组的紧密连接处也观察到 Occludin 和 Glut1 的蛋白质表达降低。还注意到白细胞-内皮细胞相互作用的促炎变化，暴露于不含尼古丁的电子烟的小鼠会损害新物体的识别性能。在此基础上得出结论，长期使用电子烟可能会对神经血管健康产生不利影响。注意到观察到的影响部分与尼古丁含量无关，尼古丁甚至可能有助于缓和非尼古丁成分对血脑屏障的影响。
更新日期：2020-08-01
中文翻译：
接触电子烟会破坏血脑屏障的完整性并促进神经炎症
自诞生以来，电子烟（e-香烟）的使用量大幅增长，尤其是在青少年和可燃烟草使用者中。几种具有已知神经血管作用的香烟烟雾成分存在于电子烟液体中或在蒸汽产生过程中形成。本研究建立了具有标准化尼古丁递送的香烟和电子烟吸入模型，然后表征对血脑屏障功能的影响。暴露后微血管 RNA 的测序揭示了在 BBB 功能中具有关键作用的几个基因的下调。在暴露后所有组的紧密连接处也观察到 Occludin 和 Glut1 的蛋白质表达降低。还注意到白细胞-内皮细胞相互作用的促炎变化，暴露于不含尼古丁的电子烟的小鼠会损害新物体的识别性能。在此基础上得出结论，长期使用电子烟可能会对神经血管健康产生不利影响。注意到观察到的影响部分与尼古丁含量无关，尼古丁甚至可能有助于缓和非尼古丁成分对血脑屏障的影响。
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