In this study, a smartphone-based quantitative dual detection mode device, integrated with gold nanoparticles (GNPs) and time-resolved fluorescence microspheres (TRFMs) lateral flow immunoassays (LFIA) for multiplex mycotoxins in cereals were established. The most frequently used visible light and fluorescence detection modes were integrated in one device. A user-friendly application was self-written to rapidly quantify results. GNPs-LFIA and TRFMs-LFIA were used to detect aflatoxin B1 (AFB1), zearalenone (ZEN), deoxynivalenol (DON), T-2 toxin (T-2), and fumonisin B1 (FB1). The visible limits of detection (vLODs) were 10/2.5/1.0/10/0.5, 2.5/0.5/0.5/2.5/0.5 μg/kg for the two methods, respectively. The quantitative limits of detection (qLODs) were 0.59/0.24/0.32/0.9/0.27, 0.42/0.10/0.05/0.75/0.04 μg/kg, respectively. The recoveries of both LFIAs ranged from 84.0%-110.0%. A parallel analysis in 30 naturally contaminated cereal samples was conducted by liquid chromatography-tandem mass spectrometry (LC-MS/MS), the results showed good consistency, indicating the practical reliability of the established methods. The developed two smartphone-based LFIAs provide a promising technique for multiplex, highly sensitive, and on-site detection of mycotoxins.

中文翻译：

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基于智能手机的双重检测模式设备，集成了两个侧向流免疫测定法，用于谷物中的多种真菌毒素。

在这项研究中，建立了一种基于智能手机的定量双重检测模式设备，该设备集成了金纳米颗粒（GNP）和时间分辨荧光微球（TRFM）横向流动免疫测定（LFIA），用于谷物中的多种真菌毒素。最常用的可见光和荧光检测模式集成在一个设备中。一个用户友好的应用程序是自编写的，可以快速量化结果。GNPs-LFIA和TRFMs-LFIA用于检测黄曲霉毒素B1（AFB1），玉米赤霉烯酮（ZEN），脱氧雪腐酚（DON），T-2毒素（T-2）和伏马菌素B1（FB1）。两种方法的可见检测极限（vLOD）分别为10 / 2.5 / 1.0 / 10 / 0.5、2.5 / 0.5 / 0.5 / 2.5 / 0.5μg/ kg。定量检出限（qLOD）分别为0.59 / 0.24 / 0.32 / 0.9 / 0.27、0.42 / 0.10 / 0.05 / 0.75 / 0.04μg/ kg。两种LFIA的回收率在84.0％-110.0％之间。通过液相色谱-串联质谱法（LC-MS / MS）对30个自然污染的谷物样品进行了平行分析，结果显示出良好的一致性，表明了所建立方法的实际可靠性。已开发的两个基于智能手机的LFIA为真菌毒素的多重，高灵敏度和现场检测提供了一种有前途的技术。