Urinary Volatile Terpenes Analyzed by Gas Chromatography-Mass Spectrometry to Monitor Breast Cancer Treatment Efficacy in Mice.,Journal of Proteome Research

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Urinary Volatile Terpenes Analyzed by Gas Chromatography-Mass Spectrometry to Monitor Breast Cancer Treatment Efficacy in Mice.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2020-03-31 , DOI: 10.1021/acs.jproteome.9b00722
Mark Woollam _{1,

2} , Meghana Teli _{1,

3} , Shengzhi Liu _{1,

3} , Ali Daneshkhah ₁ , Amanda P Siegel _{1,

2} , Hiroki Yokota _{1,

3,

4} , Mangilal Agarwal _{1,

2,

5}

Affiliation

Urinary volatile terpene (VT) levels are significantly altered with induced models of breast cancer in mice. The question arises whether VTs can detect the efficacy of antitumor treatments. BALB/c mice were injected with 4T1.2 murine tumor cells in the mammary pad or iliac artery to model localized breast cancer and induced bone metastasis. The effect of two dopaminergic antitumor agents was tested by conventional histology and altered VT levels. The headspace of urine specimens was analyzed by gas chromatography-mass spectrometry. In the localized model, the statistical significance (p < 0.05) was identified for 26% of VTs, and in the metastasis model, 19% of VTs. The authors discovered separate VT panels classifying localized/control [area under the curve (AUC) = 1.0] and metastasis/control (AUC = 0.98). Treatment samples were tested using these panels, which showed that mice treated with either agent were statistically significantly different from cancer samples, which is consistent with conventional analysis.

中文翻译：

气相色谱-质谱法分析尿挥发性萜烯以监测小鼠的乳腺癌治疗效果。

诱发小鼠乳腺癌模型后，尿中挥发性萜烯（VT）的水平发生了显着变化。出现的问题是VT是否可以检测抗肿瘤治疗的功效。向BALB / c小鼠的乳垫或动脉注射4T1.2鼠肿瘤细胞，以模拟局部乳腺癌和诱导的骨转移。两种多巴胺能抗肿瘤药的作用通过常规组织学和改变的VT水平进行了测试。尿液样品的顶空通过气相色谱-质谱分析。在局部模型中，统计学意义（p <0.05）被确定为26％的VT，在转移模型中被确定为19％的VT。作者发现了单独的VT面板，将局部/对照（曲线下面积（AUC）= 1.0）和转移/对照（AUC = 0.98）分类。

更新日期：2020-03-31

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