Sulfoxaflor Degraded by Aminobacter sp. CGMCC 1.17253 through Hydration Pathway Mediated by Nitrile Hydratase.,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Sulfoxaflor Degraded by Aminobacter sp. CGMCC 1.17253 through Hydration Pathway Mediated by Nitrile Hydratase.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-04-08 , DOI: 10.1021/acs.jafc.9b06668
Wen-Long Yang ₁ , Zhi-Ling Dai ₁ , Xi Cheng ₁ , Ling Guo ₁ , Zhi-Xia Fan ₁ , Feng Ge _{2,

3} , Yi-Jun Dai ₁

Affiliation

Sulfoxaflor, a sulfoximine insecticide, could efficiently control many insect pests of sap-feeding. Microbial degradation of sulfoxaflor and the enzymatic mechanism involved have not been studied to date. A bacterial isolate JW2 that transforms sulfoxaflor to X11719474 was isolated and identified as Aminobacter sp. CGMCC 1.17253. Both the recombinant Escherichia coli strain harboring the Aminobacter sp. CGMCC 1.17253 nitrile hydratase (NHase) gene and the pure NHase acquired sulfoxaflor-degrading ability. Aminobacter sp. CGMCC 1.17253 NHase is a typical cobalt-containing NHase content of subunit α, subunit β, and an accessory protein, and the three-dimensional homology model of NHase was built. Substrate specificity tests showed that NHase catalyzed the conversion of acetamiprid, thiacloprid, indolyl-3-acetonitrile, 3-cyanopyridine, and benzonitrile into their corresponding amides, indicating its broad substrate specificity. This is the first report of the pure bacteria degradation of the sulfoxaflor residual in the environment and reveals the enzymatic mechanism mediated by Aminobacter sp. CGMCC 1.17253.

中文翻译：

磺胺类花被氨基杆菌属物种降解。CGMCC 1.17253通过腈水合酶介导的水化途径进行。

磺胺嘧啶杀虫剂磺胺草醚可以有效地控制许多害虫的汁液喂养。迄今为止，尚未研究磺胺草醚的微生物降解及其所涉及的酶促机理。分离出了将硫代沙弗劳转变为X11719474的细菌分离株JW2，并将其鉴定为氨基杆菌。CGMCC 1.17253。两种都携带有氨基杆菌属的重组大肠杆菌菌株。CGMCC 1.17253腈水合酶（NHase）基因和纯净的NHase具有降解草甘膦的能力。氨基杆菌 CGMCC 1.17253 NHase是典型的含钴的亚基α，亚基β和辅助蛋白的NHase含量，并建立了NHase的三维同源性模型。底物特异性测试表明，NHase催化了乙酰胺，噻虫啉，吲哚基-3-乙腈，3-氰基吡啶，和苯甲腈转化为相应的酰胺，表明其广泛的底物特异性。这是纯细菌降解环境中磺胺草硫磷残留的第一份报告，并揭示了由氨基杆菌属菌介导的酶促机制。CGMCC 1.17253。

更新日期：2020-04-24

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11