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Decorating the Anammox House: Sialic Acids and Sulfated Glycosaminoglycans in the Extracellular Polymeric Substances of Anammox Granular Sludge.
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-04-09 , DOI: 10.1021/acs.est.9b07207
Marissa Boleij 1 , Hugo Kleikamp 1 , Martin Pabst 1 , Thomas R Neu 2 , Mark C M van Loosdrecht 1 , Yuemei Lin 1
Affiliation  

Anammox (anaerobic ammonium oxidation) bacteria are important for the nitrogen cycle in both natural environments and wastewater treatment plants. These bacteria have a strong tendency to grow in aggregates like biofilms and granular sludge. To understand the formation of anammox aggregates, it is required to unravel the composition of the extracellular polymeric substances (EPS), which are produced by the bacteria to develop into aggregates and granules. Here, we investigated anionic polymers in anammox granular sludge, focussing on sialic acids and sulfated glycosaminoglycans. Quantification assays and fluorescent stains indicated that sialic acids and sulfated glycosaminoglycans were present in the anammox EPS (1.6% equivalents of sialic acids and 2.4% equivalents of sulfated glycosaminoglycans). Additionally, the potential genes for the biosynthesis of sialic acids and sulfated glycosaminoglycans were analyzed in the anammox draft genomes. The finding of these components in anammox granular sludge and previously in other nonpathogenic bacteria pointed out that sialic acids and sulfated glycosaminoglycans are worth investigating in the context of a broader function in microbial communities and biofilm systems in general.

中文翻译:


装饰厌氧氨氧化房屋:厌氧氨氧化颗粒污泥胞外聚合物中的唾液酸和硫酸化糖胺聚糖。



Anammox(厌氧氨氧化)细菌对于自然环境和废水处理厂的氮循环很重要。这些细菌很容易在生物膜和颗粒污泥等聚集体中生长。为了了解厌氧氨氧化聚集体的形成,需要解开细胞外聚合物(EPS)的组成,这些物质是由细菌产生并发育成聚集体和颗粒的。在这里,我们研究了厌氧氨氧化颗粒污泥中的阴离子聚合物,重点关注唾液酸和硫酸化糖胺聚糖。定量分析和荧光染色表明,厌氧氨氧化 EPS 中存在唾液酸和硫酸化糖胺聚糖(唾液酸当量 1.6%,硫酸化糖胺聚糖当量 2.4%)。此外,在厌氧氨氧化基因组草案中分析了唾液酸和硫酸化糖胺聚糖生物合成的潜在基因。在厌氧氨氧化颗粒污泥和之前在其他非病原性细菌中发现这些成分表明,唾液酸和硫酸化糖胺聚糖在微生物群落和生物膜系统中具有更广泛的功能,值得研究。
更新日期:2020-04-23
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