In this study, we have combined the wound-healing properties of two biodegradable polymers, viz., starch and gelatin, and have reinforced their mechanical strength through cross-linking. Further, scaffolds of this polymer combination were used to support an organotypic culture of human skin for wound healing. Human dermal fibroblasts (HDFs) and human epidermal keratinocytes (HEKs) were isolated and were seeded on the scaffolds on days 1 and 7, respectively. The scaffold was then air-lifted to develop a stratified epidermal layer. Hematoxylin and eosin (H&E) staining and immunohistochemical analysis ascertained that the histology of the skin organotypic culture was similar to that of the human skin. For in vivo animal investigations, the scaffolds were transplanted in a full-thickness wound mouse model, as a one-step procedure, wherein the artificial skin substitute showed the presence of well-defined epidermis and formation of stratum basale by day 14. By combining the inherent properties of both the materials, we have synthesized a cost-effective porous scaffold with good mechanical strength and excellent biocompatibility that can be easily adapted for commercial use. The aforementioned scaffold may integrate with the surrounding tissue, accelerate wound closure, and promote tissue reorganization and remodeling.

中文翻译：

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用于全层伤口愈合的 3D 淀粉-明胶支架的体内研究

在这项研究中，我们结合了两种可生物降解聚合物，即淀粉和明胶的伤口愈合特性，并通过交联增强了它们的机械强度。此外，这种聚合物组合的支架用于支持人体皮肤的器官培养，以用于伤口愈合。分离人真皮成纤维细胞 (HDF) 和人表皮角质形成细胞 (HEK)，并分别在第 1 天和第 7 天接种在支架上。然后将支架空气提升以形成分层的表皮层。苏木精和伊红 (H&E) 染色和免疫组织化学分析确定皮肤器官培养的组织学与人类皮肤的组织学相似。对于体内在动物研究中，支架被移植到全层伤口小鼠模型中，作为一步程序，其中人工皮肤替代物在第 14 天显示出清晰的表皮和基底层的形成。通过结合固有特性在这两种材料中，我们合成了一种具有成本效益的多孔支架，具有良好的机械强度和出色的生物相容性，可以很容易地适应商业用途。上述支架可与周围组织整合，加速伤口闭合，促进组织重组和重塑。