A proper biological microenvironment conducive to tissue repair and regeneration, while the bioimplant interface directly affects the local microenvironment. In this study, to improve the biological microenvironment, a nanosized tantalum boride (Ta‐B) was coated on a titanium alloy substrate (Ti6Al4V, TC4) using magnetron cosputtering. The sample surface was characterized by X‐ray diffraction (XRD) and transmission electron microscopy (TEM). To investigate the effects of tantalum boride coating on the microenvironment, rabbit bone marrow stromal cells (BMSCs), and RAW 264.7 cells were respectively seeded on the sample surface and relevant experiments were conducted in vitro. The pure tantalum coating (Ta) and naked TC4 were prepared as controls. Our results showed that the Ta‐B coating enhanced cell proliferation and adhesion and inhibited the inflammatory response. Findings of alkaline phosphatase (ALP) staining, alizarin red staining and real‐time PCR for osteoblastic gene expression indicated that Ta‐B and Ta coating improve the osteogenesis, in which Ta‐B coating showed higher osteogenesis than Ta coating. Thus, this study suggests that Ta‐B coating with excellent biocompatibility could have new applications for wound healing in bone tissue engineering.

中文翻译：

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硼化钽作为一种生物相容性涂层，可改善生物纳米界面的成骨。

适当的生物微环境有利于组织修复和再生，而生物植入物界面直接影响局部微环境。在这项研究中，为了改善生物微环境，使用磁控共溅射将纳米尺寸的硼化钽 (Ta-B) 涂覆在钛合金基材 (Ti6Al4V, TC4) 上。样品表面通过 X 射线衍射 (XRD) 和透射电子显微镜 (TEM) 进行表征。为了研究硼化钽涂层对微环境的影响，分别在样品表面接种兔骨髓基质细胞（BMSCs）和RAW 264.7细胞，并在体外进行相关实验。制备纯钽涂层 (Ta) 和裸露的 TC4 作为对照。我们的结果表明，Ta-B 涂层增强了细胞增殖和粘附并抑制了炎症反应。碱性磷酸酶 (ALP) 染色、茜素红染色和实时 PCR 对成骨细胞基因表达的结果表明，Ta-B 和 Ta 涂层改善了成骨作用，其中 Ta-B 涂层显示出比 Ta 涂层更高的成骨作用。因此，这项研究表明，具有优异生物相容性的 Ta-B 涂层可以在骨组织工程中的伤口愈合中具有新的应用。