WRKY proteins play essential roles as negative or positive regulators of pathogen defense. This study explored the roles of different OsWRKY proteins in basal defense and Xa1-mediated resistance to Xanthomonas oryzae pv. oryzae (Xoo) infection in rice. Assays of disease in OsWRKY10_KD and OsWRKY88_KD lines following infection with an incompatible Xoo race, which induced Xa1-mediated resistance in wild-type plants, showed that OsWRKY10 and OsWRKY88 were positive regulators of Xa1-mediated resistance. OsWRKY10 also acted as a positive regulator in basal defense by directly or indirectly activating transcription of defense-related genes. OsWRKY10 activated the OsPR1a promoter by binding to specific WRKY binding sites. Two transcriptional regulatory cascades of OsWRKY10 were identified in basal defense and Xa1-mediated resistance. In the first transcriptional regulatory cascade, OsWRKY47 acted downstream of OsWRKY10 whereas OsWRKY51 acted upstream. OsWRKY10 activated OsPR1a in two distinct ways: by binding to its promoter and, at the same time, by indirect activation through OsWRKY47. In the second transcriptional regulatory cascade, OsWRKY47 acted downstream of OsWRKY10, and OsWRKY88 acted upstream. These OsWRKY10 transcriptional regulatory cascades played important roles in basal defense and Xa1-mediated resistance to enable the mounting of a rapid immune response against pathogens.

中文翻译：

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水稻中的WRKY10转录调控级联反应参与基础防御和Xa1介导的抗性。

WRKY蛋白作为病原体防御的负调节剂或正调节剂，起着至关重要的作用。这项研究探讨了不同的Os WRKY蛋白在基础防御和Xa1介导的对水稻黄单胞菌pv的抗性中的作用。米曲霉（白叶枯病）感染的水稻。在不相容的Xoo种族感染后在OsWRKY10 _KD和OsWRKY88 _KD系中进行的疾病分析表明，Os WRKY10和Os WRKY88是Xa1介导的抗性的正调控因子，在野生型植物中诱导了Xa1介导的抗性。操作系统WRKY10还通过直接或间接激活防御相关基因的转录，在基础防御中发挥积极的调节作用。Os WRKY10通过与特定的WRKY结合位点结合来激活OsPR1a启动子。Os WRKY10的两个转录调节级联被确定在基础防御和Xa1介导的抗性。在第一个转录调节级联，锇WRKY47作用的下游锇WRKY10而锇WRKY51作用上游。Os WRKY10通过两种不同的方式激活OsPR1a：与启动子结合，同时通过Os间接激活WRKY47。在第二个转录调节级联，锇WRKY47作用的下游锇WRKY10，和锇WRKY88作用上游。这些Os WRKY10转录调控级联在基础防御和Xa1介导的抗性中起着重要作用，以使针对病原体的快速免疫反应得以增强。