The effects of the secondary metabolite biosynthesis on the metabolism and morphology of the Monascus purpureus were investigated in this study. Hypha and septum length became longer after deletion of genes pigR and pksCT in M. purpureus LQ-6 by Agrobacterium tumefaciens-mediated transformation technology, highly branched hyphae, much smaller and freely dispersed mycelial pellets were observed in M. purpureus. Compared with that in the wild-type, the level of intracellular NADH and NADPH was almost constant in M. purpureus ΔpigR at 4 days, but the NADH and NADPH levels decreased by 1.58-fold and 3.71-fold in M. purpureus ΔpksCT. The present study can not only provide a kind of strategy to improve the Monascus pigments production, but also provide theoretical support for the further study of relationship between the secondary metabolites, metabolism and morphological change.

本研究研究了次生代谢产物生物合成对紫红曲霉代谢和形态的影响。菌丝和隔膜长度基因的删除之后变长猪R和PKS CT在红曲霉LQ-6通过根癌农杆菌介导的转化技术，高度支化的菌丝，小得多，并且在观察到自由分散菌丝球红曲霉。与野生型相比，紫癜支原体Δ猪R在第4天的细胞内NADH和NADPH水平几乎恒定，而NADH和NADPH水平分别降低了1.58倍和3.71倍。红曲霉Δ PKS CT。本研究不仅为提高红曲色素的产量提供了一种策略，而且为进一步研究次生代谢产物，代谢与形态变化之间的关系提供了理论支持。