In this study, the complete genomic sequence of a novel virus was determined by next-generation sequencing of a sample from a symptomatic strawberry plant with severe yellow spots and mosaic on its leaves. Its genomic organization and sequence showed that this virus is related to members of the proposed insect-specific genus "Negevirus". The sample also contained sequences from the geranium aphid Acyrthosiphon malvae. Although the virus was detected repeatedly in the same plant during the three following years, no other positive samples were obtained from the surroundings or more-distant locations. Reverse transcription qPCR analysis revealed the presence of both genomic positive and complementary negative strands of the viral genome in the sample, with a 3- to 30-fold excess of the positive strand, indicating active viral replication. As the virus was not detected in any insect species collected at this location, the virus was provisionally named "Fragaria vesca-associated virus 1" (FVaV-1).

中文翻译：

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草莓vesca相关病毒1：与negeviruses有关的新病毒。

在这项研究中，一种新型病毒的完整基因组序列是通过对有症状草莓植物的样品进行下一代测序来确定的，所述草莓植物的叶子上有严重的黄斑和花叶。它的基因组组织和序列表明，该病毒与拟议的昆虫特异性属“ Negevirus”的成员有关。该样品还含有来自天竺葵蚜虫醋虫虹鳟的序列。尽管在随后的三年中在同一棵植物中反复检测到该病毒，但没有从周围或更远的地方获得其他阳性样本。逆转录qPCR分析显示样品中存在病毒基因组的基因组正链和互补链负链，正链过量3至30倍，表明病毒复制活跃。