The globally increasing incidence of cancer, including melanoma, requires novel therapeutic strategies. Development of successful novel drugs is based on clear identification of the target mechanisms responsible for the disease progression. The specific cancer microenvironment represents a critically important aspect of cancer biology, which cannot be properly studied in simplistic cell culture conditions. Among other traditional options, the study of melanoma cell growth on the chicken chorioallantoic membrane offers several significant advantages. This model offers increased complexity compared to usual in silico culture models and still remains financially affordable. Using this model, we studied the growth of three established human melanoma cell lines: A2058, BLM, G361. The combination of histology, immunohistochemistry with the application of human-specific antibodies, intravascular injection of contrast material such as filtered Indian ink, Mercox solution and phosphotungstic acid, and X-ray micro-CT and live-cell monitoring was employed. Melanoma cells spread well on the chicken chorioallantoic membrane. However, invasion into the stroma of the chorioallantoic membrane and the limb primordium graft was rare. The melanoma cells also significantly influenced the architecture of the blood vessel network, resulting in the orientation of the vessels to the site of the tumour cell inoculation. The system of melanoma cell culture on the chorioallantoic membrane is suitable for the study of melanoma cell growth, particularly of rearrangement of the host vascular pattern after cancer cell implantation. The system also has promising potential for further development.

包括黑素瘤在内的全球癌症发病率不断增长，需要新颖的治疗策略。成功开发新药的基础是明确识别导致疾病进展的靶机制。特定的癌症微环境代表了癌症生物学的一个至关重要的方面，在简单的细胞培养条件下无法进行适当的研究。在其他传统选择中，研究鸡绒膜尿囊膜上黑色素瘤细胞的生长具有许多重要优势。与通常的计算机文化模型相比，此模型提供了更高的复杂性，并且在财务上仍然可以承受。使用该模型，我们研究了三种已建立的人类黑色素瘤细胞系的生长：A2058，BLM，G361。组织学的结合 应用人特异性抗体进行免疫组织化学，血管内注射造影剂，例如过滤后的印度墨水，Mercox溶液和磷钨酸，以及X射线微CT和活细胞监测。黑色素瘤细胞在鸡绒膜尿囊膜上很好地扩散。但是，很少侵袭绒膜尿囊膜和四肢原基移植物的基质。黑色素瘤细胞还显着影响血管网络的结构，导致血管朝向肿瘤细胞接种部位的方向。绒毛膜尿囊膜上的黑素瘤细胞培养系统适合研究黑素瘤细胞的生长，特别是癌细胞植入后宿主血管模式的重排。