Abstract Vibrio vulnificus and Vibrio parahaemolyticus are halophilic bacteria and can cause illness associated with the consumption of oysters. In this study, we tested the hypothesis to see if the adjustment of the acceleration voltage can change the efficacy of X-ray doses on V. vulnificus and V. parahaemolyticus in pure culture and inoculated V. parahaemolyticus within farm-raised live oysters (Crassostrea virginica). By using a tailor-made X-ray irradiator (Kimtron 350), the acceleration voltage was adjusted to 50, 200 or 350 kV and irradiated on pure culture of V. vulnificus or V. parahaemolyticus and farm-raised live oysters accumulated with a three-strain mixture of V. parahaemolyticus. Inactivation of V. vulnificus and V. parahaemolyticus pure cultures without filter had a lower inactivation when compared with filtered X-ray. With a 1 mm aluminum filter, 250–300 Gy X-ray reduced more than 6 log CFU ml−1 at 350 kV acceleration voltage. When 350 kV X-ray was applied on live oysters with the aluminum filter, 6 log MPN g−1 reduction of V. parahaemolyticus was achieved with 1250 Gy. The V. parahaemolyticus was significantly (p

中文翻译：

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X射线剂量对纯培养创伤弧菌和纯培养副溶血性弧菌和不同加速电压接种的养殖场养殖活牡蛎（Crassostrea virginica）的疗效

摘要 创伤弧菌和副溶血性弧菌是嗜盐菌，食用牡蛎会引起疾病。在本研究中，我们检验了这个假设，看看加速电压的调整是否可以改变 X 射线剂量对纯培养中创伤弧菌和副溶血性弧菌的疗效，并在养殖的活牡蛎中接种副溶血性弧菌（Crassostrea弗吉尼亚州）。通过使用特制的 X 射线辐照器（金创​​ 350），将加速电压调整为 50、200 或 350 kV，对创伤弧菌或副溶血弧菌的纯培养物和养殖场养殖的活牡蛎进行辐照，其中积累了三-副溶血性弧菌的菌株混合物。与过滤的 X 射线相比，无过滤器的创伤弧菌和副溶血性弧菌纯培养物的灭活率较低。使用 1 mm 铝过滤器，250–300 Gy X 射线在 350 kV 加速电压下减少了超过 6 log CFU ml-1。当 350 kV X 射线用铝过滤器应用于活牡蛎时，1250 Gy 使副溶血性弧菌减少 6 log MPN g-1。副溶血性弧菌显着（p